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Intraspecific variability of Lactarius deliciosus isolates: colonization ability and survival after cold storage

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Abstract

Intraspecific variability in root colonization, extraradical growth pattern, and survival after cold storage of Lactarius deliciosus isolates was determined in pure culture conditions using Pinus pinaster as a host plant. The ectomycorrhizal ability of L. deliciosus at 30, 45, and 60 days from inoculation was highly variable among isolates and was negatively correlated to the age of the culture (time elapsed from isolation). The formation of rhizomorphs was related to colonization ability, but no relationship was found between colonization and formation of extraradical mycelium. The final colonization achieved at 60 days from inoculation was not related to the tree species under which the sporocarps were collected. However, isolates from sporocarps collected under P. pinaster colonized more rapidly the seedlings than those collected under other pine species. The climatic range of the sporocarps from which the isolates were obtained (maritime vs. continental) was not related to the formation of mycorrhizas at 60 days from inoculation. However, isolates from sporocarps collected from a maritime climate area colonized more rapidly the P. pinaster seedlings than those collected from a continental zone. Tolerance to cold water storage of L. deliciosus was also isolate dependent. Growth revival in agar was obtained from most of the isolates after 28 months of cold storage at 4 °C, but only 10 out of 29 isolates showed unaffected growth. The ITS rDNA alignment of all the L. deliciosus isolates showed a low variability with identities over 99%. Most of the variation was detected in the ITS1 region and consisted in single nucleotide changes and/or punctual indel mutations. The number of base differences per sequence from averaging over all sequence pairs was 1.329, which is in the low range when compared with other ectomycorrhizal species. No ITS pattern due to geographical origin of the isolates could be discerned.

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Acknowledgements

The authors wish to acknowledge the financial support provided by the Instituto Nacional de Investigaciones Agroalimentarias (INIA, Spain), project RTA04-029, the Ministerio de Ciencia e Innovación de España (MICINN), project AGL2009-12884-CO3, and the European Regional Development Fund. Work of S. Hortal was financed by the Departament d’Educació i Universitats de la Generalitat de Catalunya and the European Social Fund.

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  1. Sara Hortal

    Present address: Estación Experimental de Zonas Áridas, CSIC, Ctra. de Sacramento s/n, La Cañada de San Urbano, 04120, Almería, Spain

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  1. IRTA, Patologia Vegetal, Centre de Cabrils, Ctra. Cabrils Km 2, 08348, Cabrils, Barcelona, Spain

    Javier Parladé, Sara Hortal, Herminia de la Varga & Joan Pera

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  1. Javier Parladé
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Correspondence to Javier Parladé.

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Parladé, J., Hortal, S., de la Varga, H. et al. Intraspecific variability of Lactarius deliciosus isolates: colonization ability and survival after cold storage. Mycorrhiza 21, 393–401 (2011). https://doi.org/10.1007/s00572-010-0347-x

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