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Striga seed-germination activity of root exudates and compounds present in stems of Striga host and nonhost (trap crop) plants is reduced due to root colonization by arbuscular mycorrhizal fungi

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Abstract

Root colonization by arbuscular mycorrhizal (AM) fungi reduces stimulation of seed germination of the plant parasite Striga (Orobanchaceae). This reduction can affect not only host plants for Striga, resulting in a lower parasite incidence, but also false hosts or trap crops, which induce suicidal Striga seed germination, thereby diminishing their effectiveness. In order to better understand these AM-induced effects, we tested the influence of root colonization by different AM fungi on the seed-germination activity of root exudates of the Striga hermonthica nonhost plants cowpea and cotton on S. hermonthica. We also tested the effect of AM fungi on the seed-germination activity of the Striga gesnerioides host plant cowpea on S. gesnerioides. Moreover, we studied whether mycorrhization affects the transport of seed-germination activity to above-ground plant parts. Mycorrhization not only resulted in a lower seed germination of S. gesnerioides in the presence of root exudates of the S. gesnerioides host cowpea but also seed germination of S. hermonthica was also lower in the presence of root exudates of the S. hermonthica nonhosts cowpea and cotton. Downregulation of the Striga seed-germination activity occurs not only in root exudates upon root colonization by different AM fungi but also in the compounds produced by stems. The lowered seed-germination activity does not appear to depend on the presence of seed germination inhibitors in the root exudates of mycorrhizal plants. The implication for Striga control in the field is discussed.

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References

  • Akiyama K, Hayashi H (2006) Strigolactones: chemical signals for fungal symbionts and parasitic weeds in plant roots. Ann Bot (Lond) 97:925–931. doi:10.1093/aob/mcl063

    Article  CAS  Google Scholar 

  • Berner DK, Williams OA (1998) Germination stimulation of Striga gesnerioides seeds by hosts and nonhosts. Plant Dis 82:1242–1247. doi:10.1094/PDIS.1998.82.11.1242

    Article  Google Scholar 

  • Berner DK, Schaad NW, Völksch B (1999) Use of ethylene-producing bacteria for stimulation of Striga spp. seed germination. Biol Control 15:274–282. doi:10.1006/bcon.1999.0718

    Article  Google Scholar 

  • Bouwmeester HJ, Matusova R, Zhongkui S, Beale MH (2003) Secondary metabolite signalling in host-parasitic plant interactions. Curr Opin Plant Biol 6:358–364. doi: 10.1016/S1369-5266(03) 00065-7

    Article  CAS  PubMed  Google Scholar 

  • Bouwmeester HJ, Roux C, López-Ráez JA, Bécard G (2007) Rhizosphere communication of plants, parasitic plants and AM fungi. Trends Plant Sci 12:224–230. doi:10.1016/j.tplants.2007.03.009

    Article  CAS  PubMed  Google Scholar 

  • Burleigh SH, Cavagnaro T, Jakobsen I (2002) Functional diversity of arbuscular mycorrhizas extends to the expression of plant genes involved in P nutrition. J Exp Bot 53:1593–1601. doi:10.1093/jxb/erf013

    Article  CAS  PubMed  Google Scholar 

  • CORAF (2005) Catalogue des variétés de cotonniers séléctionées. Lomé, Togo

    Google Scholar 

  • Dubé MP, Olivier A (2001) Le Striga gesnerioides et son hôte, le niébé: interaction et méthode de lutte. Can J Bot 79:1225–1240. doi:10.1139/cjb-79-10-1225

    Google Scholar 

  • Emechebe AM, Ahonsi MO (2003) Ability of excised root and stem pieces of maize, cowpea and soybean to cause germination of Striga hermonthica seeds. Crop Prot 22:347–353. doi: 10.1016/S0261-2194(02) 00177-1

    Article  Google Scholar 

  • Gbèhounou G, Adango E (2003) Trap crops of Striga hermonthica: in vitro identification and effectiveness in situ. Crop Prot 22:395–404. doi: 10.1016/S0261-2194(02) 00196-5

    Article  Google Scholar 

  • Gomez-Roldan V, Fermas S, Brewer PB, Puech-Pagès V, Dun EA, Pillot J-P, Letisse F, Matusova R, Danoun S, Portais J-C, Bouwmeester H, Bécard G, Beveridge CA, Rameau C, Rochange SF (2008) Strigolactone inhibition of shoot branching. Nature 455:189–194. doi:10.1038/nature07271

    Article  CAS  PubMed  Google Scholar 

  • Gworgwor NA, Weber HC (2003) Arbuscular mycorrhizal fungi-parasite-host interaction for the control of Striga hermonthica (Del.) Benth. in sorghum. Sorghum bicolor (L.) Moench. Mycorrhiza 13:277–281. doi:10.1007/s00572-003-0238-5

    Article  PubMed  Google Scholar 

  • Hall AE, Cissé N, Thiaw S, Elawad HOA, Ehlers JD, Ismail AM, Fery RL, Roberts PA, Kitch LW, Murdock LL, Boukar O, Phillips RD, McWatters KH (2003) Development of cowpea cultivars and germplasm by the Bean/Cowpea CRSP. Field Crops Res 82:103–134. doi:10.1016/S0378-4290(03) 00033-9

    Article  Google Scholar 

  • Kitch LW, Boukar O, Endondo C, Murdock LL (1998) Farmer acceptability criteria in breeding cowpea. Exp Agric 34:475–486. doi:10.1017/S0014479798004049

    Article  Google Scholar 

  • Koide RT, Li MG (1989) Appropriate controls for vesicular arbuscular mycorrhizal research. New Phytol 111:35–44. doi:10.1111/j.1469-8137.1989.tb04215.x

    Article  Google Scholar 

  • Lendzemo VW, Kuyper TW (2001) Effects of arbuscular mycorrhizal fungi on damage by Striga hermonthica on two contrasting cultivars of sorghum. Agric Ecosyst Environ 87:29–35. doi: 10.1016/S0167-8809(00) 00293-0

    Article  Google Scholar 

  • Lendzemo VW, Kuyper TW, Kropff MJ, Van Ast A (2005) Field inoculation with arbuscular mycorrhizal fungi reduces Striga hermonthica performance on cereal crops and has the potential to contribute to integrated Striga management. Field Crops Res 91:51–61. doi:10.1016/j.fcr.2004.05.003

    Article  Google Scholar 

  • Lendzemo VW, Kuyper TW, Matusova R, Bouwmeester HJ, Van Ast A (2007) Colonization by arbuscular mycorrhizal fungi of sorghum leads to reduced germination and subsequent attachment and emergence of Striga hermonthica. Plant Signal Behav 2:58–62

    Article  PubMed  PubMed Central  Google Scholar 

  • Mabrouk Y, Simier P, Arfaoui A, Sifi B, Delavault P, Zourgui L, Belhadj O (2007) Induction of phenolic compounds in pea (Pisum sativum L.) inoculated by Rhizobium leguminosarum and infected with Orobanche crenata. J Phytopathol 155:728–734. doi:10.1111/j.1439-0434.2007.01307.x

    Article  Google Scholar 

  • Matusova R, Van Mourik T, Bouwmeester H (2004) Changes in the sensitivity of parasitic weed seeds to germination stimulants. Seed Sci Res 14:335–344. doi:10.1079/SSR2004187

    Article  CAS  Google Scholar 

  • Newman EI (1966) A method of estimating the total length of root in a sample. J Appl Ecol 3:139–145. doi:10.2307/2401670

    Article  Google Scholar 

  • Rank C, Rasmussen LS, Jensen SR, Pierce S, Press MC, Scholes JD (2004) Cytotoxic constituents of Alectra and Striga species. Weed Res 44:265–270. doi:10.1111/j.1365-3180.2004.00398.x

    Article  CAS  Google Scholar 

  • SAS Software Release 8.2. 1990-2001. SAS Institute Inc., Cary, NC, USA

  • Sauerborn J, Kranz B, Mercer-Quarshie H (2000) Crop rotation to improve agricultural production in Sub-Saharan Africa. J Agron Crop Sci 184:67–72. doi:10.1046/j.1439-037x.2000.00368.x

    Article  Google Scholar 

  • Smith SE, Read DJ (2008) Mycorrhizal symbiosis, 3rd edn. Academic Press, London

    Google Scholar 

  • Steinkellner S, Lendzemo V, Langer I, Schweiger P, Khaosaad T, Toussaint JP, Vierheilig H (2007) Flavonoids and strigolactones in root exudates as signals in symbiotic and pathogenic plant-fungus interactions. Molecules 12:1290–1306. doi:10.3390/12071290

    Article  CAS  PubMed  Google Scholar 

  • Sun Z, Hans J, Walter MH, Matusova R, Beekwilder J, Verstapen FWA, Ming Z, Van Echtelt E, Strack D, Bisseling T, Bouwmester HJ (2008) Cloning and characterisation of a maize carotenoid cleavage dioxygenase (ZmCCD1) and its involvement in the biosynthesis of apocarotenoids with various roles in mutualistic and parasitic interactions. Planta 228:789–801. doi:10.1007/s00425-008-0781-6

    Article  CAS  PubMed  Google Scholar 

  • Umehara M, Hanada A, Yoshida S, Akiyama K, Arite T, Takeda-Kamiya N, Magome H, Kamiya Y, Shirasu K, Yoneyama K, Kyozuka J, Yamaguchi S (2008) Inhibition of shoot branching by new terpenoid plant hormones. Nature 455:195–200. doi:10.1038/nature07272

    Article  CAS  PubMed  Google Scholar 

  • Van Mele P, Van Damme P, Berner D (1992) A new technique to test germination response to Striga seeds using plants roots p. 993-999. Mededelingen. Faculteit. Landbouwwetenschappen 57(36). Universiteit van Gent

  • Van Mourik TA, Bianchi FJAA, Van der Werf W, Stomph TJ (2008) Long-term management of Striga hermonthica: strategy evaluation with a spatio-temporal population model. Weed Res 48:329–339. doi:10.1111/j.1365-3180.2008.00638.x

    Article  Google Scholar 

  • Vierheilig H, Coughlan AP, Wyss U, Piché Y (1998) Ink and vinegar; a simple staining technique for arbuscular mycorrhizal fungi. Appl Environ Microbiol 64:5004–5007

    CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgment

This work was partially funded by a grant of the Netherlands Foundation for the Advancement of Tropical Research (WOTRO) to VL.

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Authors and Affiliations

  1. Institute of Agricultural Research for Development, Maroua, P. O. Box 33, Maroua, Cameroon

    V. Lendzemo

  2. Department of Soil Quality, Wageningen University, P. O. Box 47, 6700 AA, Wageningen, The Netherlands

    T. W. Kuyper

  3. Institut für Pflanzenschutz, Universität für Bodenkultur Wien, 1190, Wien, Austria

    H. Vierheilig

  4. Departamento de Microbiología, Estación Experimental de Zaidín, CSIC, 18008, Granada, Spain

    H. Vierheilig

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  1. V. Lendzemo
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  2. T. W. Kuyper
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  3. H. Vierheilig
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Correspondence to H. Vierheilig.

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Lendzemo, V., Kuyper, T.W. & Vierheilig, H. Striga seed-germination activity of root exudates and compounds present in stems of Striga host and nonhost (trap crop) plants is reduced due to root colonization by arbuscular mycorrhizal fungi. Mycorrhiza 19, 287–294 (2009). https://doi.org/10.1007/s00572-009-0235-4

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