Skip to main content
SpringerLink
Log in

Morphological and molecular diversity and abundance of tomentelloid ectomycorrhizae in broad-leaved forests of the Hungarian Plain

  • Original Paper
  • Published:
Mycorrhiza Aims and scope Submit manuscript

Abstract

The most common representatives of tomentelloid ectomycorrhizae (EM) collected in broad-leaved forests (Populus and Quercus) of the Hungarian Plain during a 4-year project are demonstrated. Eighteen specimens of nine tomentelloid EM morphotypes were investigated. Five of these, introduced here for the first time, were characterized by microscopical–morphological and anatomical methods. Molecular identification was carried out using sequence analysis of the nrDNA ITS region. Altogether, 54 ITS sequences (6 previously published and 12 new sequences from our mycorrhizae and 36 from GenBank derived from fruitbodies) were compared by phylogenetic analyses using neighbor-joining, maximum parsimony and maximum likelihood methods. Identification to species level was successful in the case of six EM morphotypes (Tomentella galzinii, T. subtestacea, T. sublilacina, T. pilosa, T. ferruginea and T. stuposa), and the possible taxonomic position of the remaining three was approached. These results are supported by morphology, as compared with literature data. Relative abundance of the EM morphotypes within the soil samples was estimated. Our results confirm that tomentelloid EM are constant, diverse and abundant members of the EM communities in temperate-continental broad-leaved forests.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12
Fig. 13

Similar content being viewed by others

References

  • Agerer R (1990) Is there a correlation between the abundance of ectomycorrhizae and the abundance of their fruitbodies? Z Mykol 56(1):155–158

    Google Scholar 

  • Agerer R (1991) Characterization of ectomycorrhiza. In: Norris JR, Read DJ, Varma AK (eds) Techniques for the study of mycorrhiza. Methods Microbiol 23:25–73

    Google Scholar 

  • Agerer R (1995) Anatomical characteristics of identified ectomycorrhizas: an attempt towards a natural classification. In: Varma A, Hock B (eds) Mycorrhiza. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Agerer R (1996a) Ectomycorrhizae of Tomentella albomarginata (Thelephoraceae) on Scots pine. Mycorrhiza 6:1–7

    Google Scholar 

  • Agerer R (1996b) Tomentella albomarginata. In: Agerer R (ed) Colour atlas of ectomycorrhizae plate 111. Einhorn Verlag, Schwäbisch Gmünd

  • Agerer R, Klostermeyer D, Steglich W (1995) “Piceirhiza nigra” an ectomycorrhiza on Picea abies formed by a species of Thelephoraceae. New Phytol 131:377–380

    Google Scholar 

  • Agerer R, Grote R, Raidl S (2002) The new method ‘micro-mapping’, a mean to study species-specific associations and exclusions of ectomycorrhiza. Mycol Prog 1:155–166

    Google Scholar 

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    Article  CAS  PubMed  Google Scholar 

  • Bradbury SM, Danielson RM, Visser S (1998) Ectomycorrhizas of regenerating stands of lodgepole pine (Pinus contorta). Can J Bot 76:218–227

    Article  Google Scholar 

  • Brand F (1991) Ektomykorrhizen an Fagus sylvatica. Characterisierung und Identifizierung, ökologische Kennzeichnung und unsterile Kultivierung. Libri Botanici.2. IHW-Verlag

  • Brand F, Taylor AFS, Agerer R (1994) Quantitative Erfassung bekannter Ektomykorrhizen in Fichtenversuchsflächen nach behandlung mit saurer Beregnung und Kalkung. BMFT-Bericht, Bonn

    Google Scholar 

  • Corner EJH (1968) A monograph of Thelephora (Basidiomycetes). Beih Nova Hedwig 27:1–110

    Google Scholar 

  • Dahlberg A, Jonsson L, Nylund J-E (1997) Species diversity and distribution of biomass above and below ground among ectomycorrhizal fungi in an old-growth Norway spruce forest in south Sweden. Can J Bot 75:1323–1335

    Google Scholar 

  • Danielson RM, Pruden M (1989) The ectomycorrhizal status of urban spruce. Mycologia 81:335–341

    Google Scholar 

  • Erland S, Taylor AFS (1999) Resupinate ectomycorrhizal fungi. In: Cairney JWG, Chambers SM (eds) Ectomycorrhizal fungi. Key genera in profile. Springer, Berlin Heidelberg New York, pp 347–363

    Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Google Scholar 

  • Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes application to the identification of mycorrhizae and rusts. Mol Ecol 2:113–118

    CAS  PubMed  Google Scholar 

  • Gardes M, Bruns T (1996) Community structure of ectomycorrhizal fungi in a Pinus muricata forest: above- and below-ground views. Can J Bot 74:1572–1583

    Google Scholar 

  • Horton T, Bruns TD (1998) Multiple-host fungi are the most frequent and abundant ectomycorrhizal types in a mixed stand of Douglas fir (Pseudotsuga menziesii) and bishop pine (Pinus muricata). New Phytol 139:331–339

    Article  Google Scholar 

  • Jakucs E (2002) Ectomycorrhizae of Populus alba L. in South Hungary. Phyton 42:199–210

    Google Scholar 

  • Jakucs E (2002a) Tomentella pilosa. In: Agerer R (ed) Colour atlas of ectomycorrhizae plate 141. Einhorn Verlag, Schwäbisch Gmünd

  • Jakucs E (2002b) Tomentella subtestacea. In: Agerer R (ed) Colour Atlas of ectomycorrhizae plate 147. Einhorn Verlag , Schwäbisch Gmünd

  • Jakucs E, Agerer R (1999) Tomentella pilosa (Burt) Bourdot & Galzin + Populus alba L. Descr Ectomyc 4:135–140

    Google Scholar 

  • Jakucs E, Agerer R (2001) Tomentella subtestacea Bourdot & Galzin + Populus alba L. Descr Ectomyc 5:213–219

    Google Scholar 

  • Jakucs R, Agerer R, Bratek Z (1997) “Quercirhiza fibulocystidiata” + Quercus spec. Descr Ectomyc 2:67–71

    Google Scholar 

  • Jakucs R, Agerer R, Bratek Z (1998) “Quercirhiza fibulocystidiata”. In: Agerer R (ed) Colour atlas of ectomycorrhizae plate 132. Einhorn Verlag, Schwabisch Gmünd

  • Jakucs E, Kovács GM, Agerer R, Romsics C, Erős-Honti Z (2004) Morphological–anatomical characterization and molecular identification of Tomentella stuposa ectomycorrhizae and related anatomotypes. Mycorrhiza 10.1007/s00572-004-0326-1

  • Jonsson T, Kokalj S, Finlay RD, Erland S (1999) Ectomycorrhizal community structure in a limed spruce forest. Mycol Res 103:501–508

    Google Scholar 

  • Kaldorf M, Renker C, Fladung M, Buscot F (2004) Characterization and spatial distribution of ectomycorrhizas colonizing aspen clones released in an experimental field. Mycorrhiza 14:295–306

    Google Scholar 

  • Kovács GM, Bagi I (2001) Mycorrhizal status of a mixed deciduous forest from the Great Hungarian Plain with special emphasis on the potential mycorrhizal partners of Terfezia terfezioides (Matt.) Trappe. Phyton-Ann Rei Bot 41:161–168

    Google Scholar 

  • Kovács GM, Szigetvári C (2002) Mycorrhizae and other root-associated fungal structures of the plants of a sandy grassland on the Great Hungarian Plain. Phyton-Ann Rei Bot 42:211–223

    Google Scholar 

  • Köljalg U (1996) Tomentella (Basidiomycota) and related genera in Temperate Eurasia. Synopsis Fungorum. Fungiflora, Oslo

    Google Scholar 

  • Köljalg U, Dahlberg A, Taylor AFS, Larsson E, Hallenberg N, Stenlid J, Larsson K-H, Fransson PM, Karen O, Jonsson L (2000) Diversity and abundance of resupinate thelephoroid fungi as ectomycorrhizal symbionts in Swedish boreal forests. Mol Ecol 9:1985–1996

    Article  PubMed  Google Scholar 

  • Köljalg U, Jakucs E, Bóka K, Agerer R (2001) Three ectomycorrhizae with cystidia formed by different Tomentella species as revealed by rDNA IST sequences and anatomical characteristics. Folia Cryptogam Est 38:27–39

    Google Scholar 

  • Kranabetter JM, Wylie T (1998) Ectomycorrhizal community structure across forest openings on naturally regenerated western hemlock seedlings. Can J Bot 76:189–196

    Article  Google Scholar 

  • Larsen MJ (1974) A contribution to the taxonomy of the genus Tomentella. Mycol Mem 4:1–145

    Google Scholar 

  • Mehmann B, Egli S, Braus GH, Brunner I (1995) Coincidence between molecularly and morphologically classified ectomycorrhizal morphotypes and fruitbodies in a spruce forest. In: Stocchi V, Bonfante, Nuti M (eds) Biotechnology of ectomycorrhizae. Plenum, New York, pp 229–239

    Google Scholar 

  • Ozinga WA, van Andel J, McDonnel AMP (1997) Nutritional soil heterogeneity and mycorrhiza as determinants of plant species diversity. Acta Bot Neerl 46(3):237–254

    Google Scholar 

  • Page RDM (1996) TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358

    CAS  PubMed  Google Scholar 

  • Palfner G, Agerer R (1996) “Quercirhiza squamosa” eine nichtidentifizierte Ektomykorrhiza an Quercus robur. Sendtnera 3:137–145

    Google Scholar 

  • Pigott CD (1982) Survival of mycorrhiza formed by Cenococcum geophilum Fr. in dry soils. New Phytol 92:513–517

    Google Scholar 

  • Posada D, Crandall KA (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics 14:817–818

    Article  CAS  PubMed  Google Scholar 

  • Raidl S (1997) Studien zur Ontogenie an Rhizomorphen von Ektomycorrhizen. Bibl Mycol 169:1–184. Cramer, Berlin

    Google Scholar 

  • Raidl S (1998) Tomentella ferruginea. In: Agerer R (ed) Colour atlas of ectomycorrhizae plate 137. Einhorn Verlag, Schwäbisch Gmünd

  • Raidl S, Müller WR (1996) Tomentella ferruginea (Pers.) Pat. + Fagus sylvatica L. Descr Ectomyc 1:161–166

    Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Staden R, Beal KF, Bonfield JK (2000) The Staden package, 1998. Methods Mol Biol 132:115–130

    Google Scholar 

  • Stalpers JA (1993) The Aphyllophoraceous fungi I. Keys to the species of the Thelephorales. Stud Mycol 35:1–168

    Google Scholar 

  • Swofford DL (2003) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sinauer, Sunderland, MA

    Google Scholar 

  • Taylor DL, Bruns TD (1997) Independent, specialized invasions of ectomycorrhizal mutualism by two nonphotosynthetic orchids. Proc Natl Acad Sci U S A 94:4510–4515

    Google Scholar 

  • Taylor DL, Bruns TD (1999) Community structure of ectomycorrhizal fungi in a Pinus muricata forest: minimal overlap between the mature forest and resistant propagule communities. Mol Ecol 8:1837–1850

    Article  CAS  PubMed  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882

    Article  Google Scholar 

  • Visser S (1995) Ectomycorrhizal succession in jack pine stands following wildfire. New Phytol 129:389–401

    Google Scholar 

Download references

Acknowledgements

This study was supported by the Hungarian Research Fund (OTKA), grant no. T 038031 and D 048333. G.M. Kovács is a grantee of the Bolyai János Scholarship. We thank Dr. Reinhard Agerer and Dr. Stefan Raidl for consultation about the microscopic preparations, Dr. Balázs Harrach and Dr. Mária Benkő for providing facilities for molecular work and Piroska Dózsainé Kerekes for laboratory assistance.

Author information

Authors and Affiliations

  1. Department of Plant Anatomy, Eötvös Loránd University, Pázmány Péter sétány 1/c, 1117, Budapest, Hungary

    Erzsébet Jakucs, Gábor M. Kovács, Gyöngyi Szedlay & Zsolt Erős-Honti

  2. Veterinary Medical Research Institute, Hungarian Academy of Sciences, P.O. Box 18, 1581, Budapest, Hungary

    Gábor M. Kovács

Authors
  1. Erzsébet Jakucs
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gábor M. Kovács
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gyöngyi Szedlay
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zsolt Erős-Honti
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Erzsébet Jakucs.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jakucs, E., Kovács, G.M., Szedlay, G. et al. Morphological and molecular diversity and abundance of tomentelloid ectomycorrhizae in broad-leaved forests of the Hungarian Plain. Mycorrhiza 15, 459–470 (2005). https://doi.org/10.1007/s00572-005-0351-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00572-005-0351-8

Keywords

Search

Navigation