Abstract
Morphological and molecular characters were analysed to investigate diversity within isolates of the Glomus claroideum/Glomus etunicatum species group in the genus Glomus. The inter- and intra-isolate sequence diversity of the large subunit (LSU) rRNA gene D2 region of eight isolates of G. claroideum and G. etunicatum was studied using PCR-single strand conformational polymorphism (SSCP)-sequencing. In addition, two isolates recently obtained from Southern China were included in the analysis to allow for a wider geographic screening. Single spore DNA isolation confirmed the magnitude of gene diversity found in multispore DNA extractions. An apparent overlap of spore morphological characters was found between G. claroideum and G. etunicatum in some isolates. Analysis of the sequence frequencies in all G. etunicatum and G. claroideum isolates (ten) showed that four LSU D2 sequences, representing 32.1% of the clones analysed for multispore extraction (564) were found to be common to both species, and those sequences were the most abundant in four of the ten isolates analysed. The frequency of these sequences ranged between 23.2% and 87.5% of the clones analysed in each isolate. The implications for the use of phenotypic characters to define species in arbuscular mycorrhizal fungi are discussed. The current position of G. claroideum/G.etunicatum in the taxonomy of the Glomeromycota is also discussed.
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Acknowledgements
This work was partly supported by funds contributed from the following sources: The International Institute of Biotechnology (IIB), the BEGNET project EU Framework IV, contract number BIO4-CT97-2225 and NATO grant ENVIR.LG.950923. Alia Rodriguez wishes to express her thanks to COLFUTURO, Carrera 15 No. 37–15, Santafe de Bogota (Colombia), Research School of Biosciences, University of Kent and IIB for the financial support of her Ph.D. studies. The authors would like to thank Drs. David Sylvia, Miroslav Vosatka and Mauritz Vestberg for providing some of the isolates included in this study. Professors Xiaolin Li (China Agricultural University, Beijing), Bin Zhao (Hauzhong Agricultural University, Wuhan) and Yuen Ha Wong (Hong Kong Polytechnic University, Hong Kong, P.R. China) are also acknowledged for the provision of original Chinese soil samples or cultures. The Mychintec (ICA4-CT-2000-30014) project (partner IIB) from the EU INCO-DEV FP5 programme supported Louisa Robinson and the sequencing of Chinese isolates of AMF.
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Rodriguez, A., Clapp, J.P., Robinson, L. et al. Studies on the diversity of the distinct phylogenetic lineage encompassing Glomus claroideum and Glomus etunicatum . Mycorrhiza 15, 33–46 (2005). https://doi.org/10.1007/s00572-003-0291-0
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DOI: https://doi.org/10.1007/s00572-003-0291-0