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Variation in nitrogen source utilisation by nine Amanita muscaria genotypes from Australian Pinus radiata plantations

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Abstract

The abilities of nine genotypes of Amanita muscaria (L.:Fr) Pers. to utilise a range of inorganic and organic nitrogen sources for growth was examined in axenic liquid cultures. Considerable intraspecific variation was observed in biomass yields on all substrates; however biomass yield was highest on glutamine and/or NH4 + for all genotypes. Yields on aspartic acid, glutamic acid and histidine were generally low relative to NH4 +, while utilisation of arginine and glycine showed marked variation between genotypes. Eight genotypes produced significantly less biomass on bovine serum albumin than on NH4 +, raising questions regarding classification of A. muscaria as a 'protein fungus'.

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References

  • Abuzinadah RA, Read DJ (1986) The role of proteins in the nitrogen nutrition of ectomycorrhizal plants. I. Utilisation of peptides and proteins by ectomycorrhizal fungi. New Phytol 103:481–493

    CAS  Google Scholar 

  • Abuzinadah RA, Read DJ (1988) Amino acids as nitrogen sources for ectomycorrhizal fungi: utilisation of individual amino acids. Trans Br Mycol Soc 91:473–479

    CAS  Google Scholar 

  • Anderson IC, Chambers SM, Cairney JWG (1999) Intra- and interspecific variation in patterns of organic and inorganic nitrogen utilization by three Australian Pisolithus species. Mycol Res 103:1579–1587

    Article  Google Scholar 

  • Anderson IC, Chambers SM, Cairney JWG (2001) Variation in nitrogen source utilisation by Pisolithus isolates maintained in axenic culture. Mycorrhiza 11:53–56

    Article  CAS  Google Scholar 

  • Bougher NL (1996) Diversity of ectomycorrhizal fungi associated with eucalypts in Australia. In: Brundrett M, Dell B, Malajczuk N, Minquin G (eds) Mycorrhizas for plantation forestry in Asia. ACIAR, Canberra, pp 8–15

  • Buscot F, Munch JC, Charcosset JY, Gardes M, Nehls U, Hampp R (2000) Recent advances in exploring physiology and biodiversity of ectomycorrhizas highlight the functioning of these symbioses in ecosystems. FEMS Microbiol Rev 24:601–614

    Google Scholar 

  • Cairney JWG (1999) Intraspecific physiological variation: implications for understanding functional diversity in ectomycorrhizal fungi. Mycorrhiza 9:125–135

    Article  Google Scholar 

  • Cairney JWG, Sawyer NA, Sharples JM, Meharg AA (2000) Intraspecific variation in nitrogen source utilisation by isolates of the ericoid mycorrhizal fungus Hymenoscyphus ericae (Read) Korf and Kernan. Soil Biol Biochem 32:1319–1322

    Article  CAS  Google Scholar 

  • Chalot M, Brun A (1998) Physiology of organic nitrogen acquisition by ectomycorrhizal fungi and ectomycorrhizas. FEMS Microbiol Rev 22:21–44

    Article  PubMed  Google Scholar 

  • Connell MJ, Raison RJ, Khanna PK (1995) Nitrogen mineralisation in relation to site history and soil properties for a range of Australian forest soils. Biol Fertil Soils 20:213–220

    Google Scholar 

  • Dickie IA, Koide RT, Stevens CM (1998) Tissue density and growth response of ectomycorrhizal fungi to nitrogen source and concentration. Mycorrhiza 8:145–148

    Article  Google Scholar 

  • Finlay RD, Frostegård Å, Sonnerfeldt A-M (1992) Utilisation of organic and inorganic nitrogen sources by ectomycorrhizal fungi in pure culture and in symbiosis with Pinus contorta Dougal. Ex Loud. New Phytol 120:105–115

    Google Scholar 

  • France RC, Reid CPP (1984) Pure culture growth of ectomycorrhizal fungi on inorganic nitrogen sources. Microbial Ecol 10:187–195

    Google Scholar 

  • Keller G (1996) Utilisation of inorganic and organic nitrogen sources by high-subalpine ectomycorrhizal fungi of Pinus cembra in pure culture. Mycol Res 100:989–998

    CAS  Google Scholar 

  • Littke WR, Bledsoe CS, Edmonds RL (1984) Nitrogen uptake and growth in vitro by Hebeloma crustuliniforme and other Pacific Northwest mycorrhizal fungi. Can J Bot 62:647–652

    CAS  Google Scholar 

  • Marx DH, Bryan WC (1975) Growth and ectomycorrhizal development of loblolly pine seedlings in fumigated soil infested with the fungal symbiont Pisolithus tinctorius. For Sci 21:245–254

    Google Scholar 

  • Nehls U, Kleber R, Wiese J, Hampp R (1999) Isolation and characterization of a general amino acid permease from the ectomycorrhizal fungus Amanita muscaria. New Phytol 144:343–349

    Article  CAS  Google Scholar 

  • Nehls U, Bock A, Einig W, Hampp R (2001) Excretion of two proteases by the ectomycorrhizal fungus Amanita muscaria. Plant Cell Environ 24:741–747

    Google Scholar 

  • Rangel-Castro JI, Danell E, Taylor AFS (2002) Use of different nitrogen sources by the edible ectomycorrhizal mushroom Cantharellus cibarius. Mycorrhiza 12:131–137

    Article  CAS  PubMed  Google Scholar 

  • Sangtiean T, Schmidt S (2002) Growth of subtropical ECM fungi with different nitrogen sources using a new floating culture technique. Mycol Res 106:74–85

    Article  CAS  Google Scholar 

  • Sawyer NA, Chambers SM, Cairney JWG (2001) Distribution and persistence of Amanita muscaria genotypes in Australian Pinus radiata plantations. Mycol Res 105:966–970

    CAS  Google Scholar 

  • Smith SE, Read DJ (1997) Mycorrhizal symbiosis. Academic, London

  • Taylor AFS, Martin F, Read DJ (2000) Fungal diversity in ectomycorrhizal communities of Norway spruce [Picea abies (L.) Karst.] and beech (Fagus sylvatica L.) along north-south transects in Europe. In: Schulze E-D (ed) Carbon and nitrogen cycling in European forest ecosystems (Ecological Studies, vol 142). Springer, Berlin Heidelberg New York, pp 343–365

  • Yang ZL, Weiß M, Kottke I, Oberwinkler F, Nehls U, Guttenberger M, Hampp R (1999) Amanita. In: Cairney JWG, Chambers SM (eds) Ectomycorrhizal fungi: key genera in profile. Springer, Berlin Heidelberg New York, pp 201–230

    Google Scholar 

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Correspondence to John W. G. Cairney.

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Sawyer, N.A., Chambers, S.M. & Cairney, J.W.G. Variation in nitrogen source utilisation by nine Amanita muscaria genotypes from Australian Pinus radiata plantations. Mycorrhiza 13, 217–221 (2003). https://doi.org/10.1007/s00572-003-0229-6

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  • DOI: https://doi.org/10.1007/s00572-003-0229-6

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