Abstract
In this research, we present a simple and cost effective soft lithographic process to fabricate polylactic acid (PLA) scaffolds for tissue engineering. In which, the negative photoresist JSR THB-120N was spun on a glass subtract followed by conventional UV lithographic processes to fabricate the master to cast the PDMS elastomeric mold. A thin poly(vinyl alcohol) (PVA) layer was used as a mode release such that the PLA scaffold can be easily peeled off. The PLA precursor solution was then cast onto the PDMS mold to form the PLA microstructures. After evaporating the solvent, the PLA microstructures can be easily peeled off from the PDMS mold. Experimental results show that the desired microvessels scaffold can be successfully transferred to the biodegradable polymer PLA. Encouraging progress in bovine endothelial cells seeding was observed.
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Acknowledgments
The authors would like to thank the National Science Council of Taiwan, for financially supporting this work under Contract No. NSC-96-2212-E-005-024. The Center of Nanoscience and Nanotechnology at National Chung-Hsing University, Taiwan, is appreciated for use of its facilities.
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Wang, GJ., Ho, KH. & Hsueh, CC. Biodegradable polylactic acid microstructures for scaffold applications. Microsyst Technol 14, 989–993 (2008). https://doi.org/10.1007/s00542-007-0482-2
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DOI: https://doi.org/10.1007/s00542-007-0482-2