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Direct effects of Rho-kinase inhibitor on pial microvessels in rabbits

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Abstract

Purpose

Rho-kinase inhibitor is widely used for prevention of cerebral vascular spasm. However, the cerebral pial vascular action of Rho-kinase inhibitor has not been investigated. We therefore evaluated the direct effects of Y-27632, a Rho-kinase inhibitor, on pial microvessels.

Method

Experiments were performed on anesthetized rabbits. A closed cranial window was used to visualize the pial microcirculation. After baseline hemodynamic and pial vascular measurements, the cranial window was superfused with four increasing concentrations of Y-27632 (10−9, 10−7, 10−6, 10−5 mol l−1; n = 7) dissolved in artificial cerebrospinal fluid for 7 min each. We measured the diameters of pial vessels, mean arterial pressure (MAP), heart rate (HR), and rectal temperature at 7 min after application of each Y-27632 concentration.

Results

MAP, HR, rectal temperature, arterial pH, PaCO2, PaO2, and plasma Na+, K+ and glucose concentrations did not change significantly during the experimental period. Y-27632 at 10−9 to 10−7 mol l−1 did not produce any significant change in pial arterioles. Topical application of Y-27632 at 10−6 and 10−5 mol l−1 produced pial large (8.4 ± 5.7 and 19.8 ± 12.7 %) and small (10.1 ± 8.5 and 18.1 ± 12.3 %) arterioles dilation. However, Y-27632 did not produce any change in pial large and small venules.

Conclusion

We evaluated the direct effects of Y-27632 on pial microvessels. Y-27632 dilates only pial arterioles in a concentration-dependent manner, and most at a concentration of 10−5 mol l−1. Y-27632 is a potent cerebral pial arteriolar dilator but is not a venular dilator.

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Acknowledgments

This work was supported by JSPS KAKENHI Grant Numbers 19591784 and 23592246.

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Authors and Affiliations

  1. Department of Anesthesiology, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409-3898, Japan

    Masakazu Kotoda & Takashi Matsukawa

  2. Surgical Center, University of Yamanashi Hospital, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409-3898, Japan

    Tadahiko Ishiyama & Noriyuki Shintani

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  1. Masakazu Kotoda
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  2. Tadahiko Ishiyama
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  4. Takashi Matsukawa
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Correspondence to Masakazu Kotoda.

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Kotoda, M., Ishiyama, T., Shintani, N. et al. Direct effects of Rho-kinase inhibitor on pial microvessels in rabbits . J Anesth 29, 186–190 (2015). https://doi.org/10.1007/s00540-014-1903-x

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  • DOI: https://doi.org/10.1007/s00540-014-1903-x

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