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Urinary trypsin inhibitor ameliorates renal tissue oxygenation after ischemic reperfusion in rats

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Abstract

Purpose

In order to determine the mechanism of the protective effect of a urinary trypsin inhibitor (UTI) on renal ischemic reperfusion injury, we measured the tissue oxygen partial pressure (\( p_{O_2 } \)) in both the renal cortex and medulla in rats, using electron paramagnetic resonance (EPR) oximetry.

Methods

We allocated the rats to three groups: normal saline (NS) group, a UTI 50 000 U·kg−1 (LD) group, and a UTI 150 000 U·kg−1 (HD) group, with the normal saline and UTI being administered 30 min before ischemia. Renal ischemia was achieved by inflating the balloon of a vascular occluder that had been placed around the abdominal aorta just above the bifurcation of the renal artery. Cortical and medullary \( p_{O_2 } \) were measured every 10 min during ischemia (30 min) and reperfusion (60 min) by EPR oximetry; also, systemic cardiopulmonary parameters were measured.

Results

The \( p_{O_2 } \) in the cortex and medulla decreased to less than 2 mmHg during ischemia in all groups. At 60 min after reperfusion, the \( p_{O_2 } \) values in the NS group were not fully restored, whereas those in the LD and HD groups were completely restored to the pre-ischemic values. There were no significant differences between the HD and LD groups. There were no differences between any groups in cardiopulmonary parameters.

Conclusion

Because UTI improved renal oxygenation after reperfusion without changing cardiopulmonary parameters, the pharmacological properties of UTI, such as its renal protection and anti-shock activity, may be explained in part, by this improvement in tissue oxygenation.

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Authors and Affiliations

  1. Department of Anesthesiology and Emergency Medicine, Kagawa University, School of Medicine, 1750-1 Ikenobe, Miki-cho, Kagawa, 761-0793, Japan

    Satoshi Taie, Masaaki Ueki, Kosuke Chujo, Takehiko Asaga, Yasuyuki Iwanaga, Junichiro Ono & Nobuhiro Maekawa

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  1. Satoshi Taie
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  2. Masaaki Ueki
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  3. Kosuke Chujo
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  4. Takehiko Asaga
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  5. Yasuyuki Iwanaga
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  6. Junichiro Ono
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  7. Nobuhiro Maekawa
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Taie, S., Ueki, M., Chujo, K. et al. Urinary trypsin inhibitor ameliorates renal tissue oxygenation after ischemic reperfusion in rats. J Anesth 22, 149–154 (2008). https://doi.org/10.1007/s00540-007-0602-2

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  • DOI: https://doi.org/10.1007/s00540-007-0602-2

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