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Unilateral adrenalectomy attenuates hemorrhagic shock-induced analgesia in rats

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Abstract

Purpose

To assess the importance of the pituitary adrenal axis in producing stress-induced analgesia (SIA) after hemorrhagic shock, we performed formalin tests after hemorrhage and reinfusion in unilaterally adrenalectomized or sham-operated rats.

Methods

Fifty-two adult Sprague-Dawley rats were divided into seven groups: sham-operation normotensive (n = 8), sham-operation shock (n = 8), adrenalectomy normotensive (n = 7), adrenalectomy shock (n = 7), sham-operation shock + yohimbine (n = 7), sham-operation normotensive + corticosterone (n = 7), and adrenalectomy shock + corticosterone (n = 8). The left adrenal gland was cauterized 24 h before the experiment. The mean blood pressure in the shock groups was kept at 50–60 mmHg for 30 min by draining arterial blood. After the blood-reinfusion or observation period, 10% formalin was injected into the rear paw. Nociceptive behaviors and locomotion were observed and rated for 1 h, using the criteria of Dubuisson and Dennis. In 12 other sham-operated and adrenalectomized rats, plasma adrenalin, noradrenalin, and corticosterone concentrations were measured before and after hemorrhagic shock.

Results

Although the sham-operation shock group showed a lower pain score, the adrenalectomy shock group showed nociceptive behavior similar to that in the normotensive groups. Yohimbine did not affect the SIA; however, corticosterone administration reversed the effects of the adrenalectomy on the SIA. The plasma corticosterone levels in the unilaterally adrenalectomized rats were lower than those in the sham-operated rats and did not increase after hemorrhagic shock.

Conclusion

These results suggest that adrenocortical systems play an important role in hemorrhagic shock-induced SIA.

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References

  1. HK Beecher (1946) ArticleTitlePain in man wounded in battle Ann Surg 123 96–105 Occurrence Handle17858731 Occurrence Handle10.1097/00000658-194601000-00008 Occurrence Handle1:STN:280:DC%2BD2srktlyjsw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  2. RJ Bodnar DD Kelly M Brutus M Glusman (1980) ArticleTitleStress-induced analgesia: neural and hormonal determinants Neurosci Biobehav Rev 4 87–100 Occurrence Handle6995874 Occurrence Handle10.1016/0149-7634(80)90028-7 Occurrence Handle1:CAS:528:DyaL3cXkt1eiu7g%3D

    Article  PubMed  CAS  Google Scholar 

  3. GW Terman Y Shavit JW Lewis JT Cannon JC Liebeskind (1984) ArticleTitleIntrinsic mechanisms of pain inhibition: activation by stress Science 226 1270–1277 Occurrence Handle6505691 Occurrence Handle10.1126/science.6505691 Occurrence Handle1:STN:280:BiqD28rnslY%3D

    Article  PubMed  CAS  Google Scholar 

  4. T Fukuda Nishimoto Ch M Miyabe H Toyooka (2001) ArticleTitleThe residual effects of hemorrhagic shock on pain reaction and c-fos expression in rats Anesth Analg 93 424–429 Occurrence Handle11473874 Occurrence Handle10.1097/00000539-200108000-00038 Occurrence Handle1:STN:280:DC%2BD3MvislSktQ%3D%3D

    Article  PubMed  CAS  Google Scholar 

  5. T Fukuda Ch Nishimoto H Toyooka (2005) ArticleTitleEffects of naloxone on stress-induced analgesia following hemorrhagic shock Reg Anesth Pain Med 30 339–343 Occurrence Handle16032585 Occurrence Handle10.1016/j.rapm.2005.03.013 Occurrence Handle1:CAS:528:DC%2BD2MXmsVOkurg%3D

    Article  PubMed  CAS  Google Scholar 

  6. S Mousa CH Miller D Couri (1981) ArticleTitleCorticosteroid modulation and stress-induced analgesia in rats Neuroendocrinology 33 317–319 Occurrence Handle7301053 Occurrence Handle1:CAS:528:DyaL38XhsFChug%3D%3D

    PubMed  CAS  Google Scholar 

  7. RJ Bodnar NS Sharpless JH Kordower M Potegal GA Barr (1982) ArticleTitleAnalgesia responses following adrenal demedullation and peripheral catecholamine depletion Physiol Behav 29 1105–1109 Occurrence Handle6819590 Occurrence Handle10.1016/0031-9384(82)90305-5 Occurrence Handle1:CAS:528:DyaL3sXislylsw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  8. DD Kelly AJ Silverman M Glusman RJ Bodnar (1993) ArticleTitleCharacterization of pituitary mediation of stress-induced antinociception in rats Physiol Behav 53 769–775 Occurrence Handle8511184 Occurrence Handle10.1016/0031-9384(93)90187-K Occurrence Handle1:STN:280:ByyB1cfnt1M%3D

    Article  PubMed  CAS  Google Scholar 

  9. LC Sutton M Fleshner R Mazzeo SF Maier LR Watkins (1994) ArticleTitleA permissive role of corticosterone in an opioid form of stress-induced analgesia: blockade of opiate analgesia is not due to stress-induced hormone release Brain Res 663 19–29 Occurrence Handle7850467 Occurrence Handle10.1016/0006-8993(94)90458-8 Occurrence Handle1:CAS:528:DyaK2MXitFOnsLw%3D

    Article  PubMed  CAS  Google Scholar 

  10. AA Filaretov AI Bogdanov NI Yarushkina (1996) ArticleTitleStress-induced analgesia. The role of hormones produced by the hypophyseal-adrenocortical system Neurosci Behav Physiol 26 572–578 Occurrence Handle9121636 Occurrence Handle10.1007/BF02359502 Occurrence Handle1:STN:280:ByiC2szovVc%3D

    Article  PubMed  CAS  Google Scholar 

  11. F Sierralta D Naquira G Pinardi HF Miranda (1996) ArticleTitleα-Adrenoceptor and opioid receptor modulation of clonidine-induced antinociception Br J Pharmacol 119 551–554 Occurrence Handle8894177 Occurrence Handle1:CAS:528:DyaK28XmtFyru7Y%3D

    PubMed  CAS  Google Scholar 

  12. HB Lee MD Blaufox (1985) ArticleTitleBlood volume in the rat J Nucl Med 25 72–76

    Google Scholar 

  13. D Dubuisson SG Dennis (1977) ArticleTitleThe formalin test: a quantitative study of the analgesic effects of morphine, meperidine, and brain stem stimulation in rats and cats Pain 4 161–174 Occurrence Handle564014 Occurrence Handle10.1016/0304-3959(77)90130-0 Occurrence Handle1:CAS:528:DyaE1cXhsVGrt7s%3D

    Article  PubMed  CAS  Google Scholar 

  14. R Guillemin T Vargo J Rossier S Minick N Ling C Rivier W Vale F Bloom (1977) ArticleTitleβ-Endorphin and adrenocorticotropin are secreted concomitantly by the pituitary gland Science 197 1367–1369 Occurrence Handle197601 Occurrence Handle10.1126/science.197601 Occurrence Handle1:CAS:528:DyaE2sXlvVWnt7w%3D

    Article  PubMed  CAS  Google Scholar 

  15. AM Aloisi ME Albonetti M Muscettola F Facchinetti C Tanganelli G Carli (1995) ArticleTitleEffects of formalin-induced pain on ACTH, beta-endorphin, corticosterone and interleukin-6 plasma levels in rats Neuroendocrinology 62 13–18 Occurrence Handle7566433 Occurrence Handle1:CAS:528:DyaK2MXnt1Sltb8%3D

    PubMed  CAS  Google Scholar 

  16. BK Taylor SF Akana MA Peterson MF Dallman AI Basbaum (1998) ArticleTitlePituitary-adrenocortical responses to persistent noxious stimuli in the awake rat: endogenous corticosterone does not reduce nociception in the formalin test Endocrinology 139 2407–2413 Occurrence Handle9564852 Occurrence Handle10.1210/en.139.5.2407 Occurrence Handle1:CAS:528:DyaK1cXivFShtbw%3D

    Article  PubMed  CAS  Google Scholar 

  17. TJ Coderre ME Fundytus JE McKenna S Dalal R Melzack (1993) ArticleTitleThe formalin test: a validation of the weighted-scores method of behavioural pain rating Pain 54 43–50 Occurrence Handle8378102 Occurrence Handle10.1016/0304-3959(93)90098-A Occurrence Handle1:STN:280:ByyA1M%2FpvVU%3D

    Article  PubMed  CAS  Google Scholar 

  18. FV Abott KBJ Franklin RF Westbrook (1995) ArticleTitleThe formalin test: scoring properties of the first and second phases of the pain response in rats Pain 60 91–102 Occurrence Handle10.1016/0304-3959(94)00095-V

    Article  Google Scholar 

  19. M Jhanwar-Uniyal KJ Renner MT Bailo VN Luine SF Leibowitz (1989) ArticleTitleCorticosterone-dependent alterations in utilization of catecholamines in discrete areas of rat brain Brain Res 500 247–255 Occurrence Handle2605494 Occurrence Handle10.1016/0006-8993(89)90320-X Occurrence Handle1:CAS:528:DyaK3cXjtVOltA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  20. G Telegdy I Vermes (1975) ArticleTitleEffect of adrenocortical hormones on activity of the serotoninergic system in limbic structures in rats Neuroendocrinology 18 16–26 Occurrence Handle1143627 Occurrence Handle1:CAS:528:DyaE2MXlsFygsrY%3D

    PubMed  CAS  Google Scholar 

  21. A Cintra K Fuxe V Solfrini LF Agnati B Tinner AC Wikstrom W Staines S Okret JA Gustafsson (1991) ArticleTitleCentral peptidergic neurons as targets for glucocorticoid action. Evidence for the presence of glucocorticoid receptor immunoreactivity in various types of classes of peptidergic neurons J Steroid Biochem Mol Biol 40 93–103 Occurrence Handle1683565 Occurrence Handle10.1016/0960-0760(91)90172-2 Occurrence Handle1:CAS:528:DyaK38XjsVOltA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  22. BS McEwen ER DeKloet W Rostene (1986) ArticleTitleAdrenal steroid receptors and actions in the nervous system Physiol Rev 66 1121–1188 Occurrence Handle3532143 Occurrence Handle1:CAS:528:DyaL2sXmsFI%3D

    PubMed  CAS  Google Scholar 

  23. NI Iarushkina AI Bogdanov (1998) ArticleTitleThe role of corticosteroids in analgesic effect caused by stimulation of the periaqueductal gray matter of the midbrain in rats Ross Fiziol Zh Im I M Sechenova 84 642–650 Occurrence Handle9805776 Occurrence Handle1:STN:280:DyaK1M%2Fit1Kiug%3D%3D

    PubMed  CAS  Google Scholar 

  24. AA Filaretov AI Bogdanov NI Yarushkina (1990) ArticleTitleAntinociceptive effect of midbrain periaqueductal gray matter (PAG) stimulation and pituitary-adrenocortical system (PACS) Neuroendocrinol Lett 12 326

    Google Scholar 

  25. ZY Hu E Bourreau I Jung-Testas P Robel EE Baulieu (1987) ArticleTitleNeurosteroids: oligodendrocyte mitochondria convert cholesterol to pregnenolone Proc Natl Acad Sci USA 84 8215–8219 Occurrence Handle3479785 Occurrence Handle10.1073/pnas.84.23.8215 Occurrence Handle1:STN:280:BieD28fls1A%3D

    Article  PubMed  CAS  Google Scholar 

  26. I Jung-Testas ZY Hu EE Baulieu P Robel (1989) ArticleTitleNeurosteroids: biosynthesis of pregnenolone and progesterone in primary cultures of rat glial cells Endocrinology 93 1157–1162

    Google Scholar 

  27. O Zinder DE Dar (1999) ArticleTitleNeuroactive steroids: their mechanism of action and their function in the stress response Acta Physiol Scand 167 181–188 Occurrence Handle10606819 Occurrence Handle10.1046/j.1365-201x.1999.00579.x Occurrence Handle1:CAS:528:DC%2BD3cXhslWlug%3D%3D

    Article  PubMed  CAS  Google Scholar 

  28. MD Majewska NL Harrison RD Schwartz JL Barker SM Paul (1986) ArticleTitleSteroid hormone metabolites are barbiturate-like modulators of the GABA receptor Science 232 1004–1007 Occurrence Handle2422758 Occurrence Handle10.1126/science.2422758 Occurrence Handle1:CAS:528:DyaL28XktVSqsr0%3D

    Article  PubMed  CAS  Google Scholar 

  29. ML Barbaccia G Roscetti M Trabucchi MC Mostallino A Concas RH Purdy G Biggio (1996) ArticleTitleTime-dependent changes in rat brain neuroactive steroid concentrations and GABAa receptor function after acute stress Neuroendocrinology 63 166–172 Occurrence Handle9053781 Occurrence Handle1:CAS:528:DyaK28XitVaitrg%3D

    PubMed  CAS  Google Scholar 

  30. RH Purdy AL Morrow PH Moore SM Paul (1991) ArticleTitleStress-induced elevations of γ-aminobutyric acid type A receptor-active steroids in the rat brain Proc Natl Acad Sci USA 88 4553–4557 Occurrence Handle1852011 Occurrence Handle10.1073/pnas.88.10.4553 Occurrence Handle1:CAS:528:DyaK3MXkvVGqt7g%3D

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Anesthesiology, Graduate School of Comprehensive Human Sciences, Tsukuba University, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan

    Taeko Fukuda, Chikako Nishimoto, Masayuki Miyabe & Hidenori Toyooka

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  1. Taeko Fukuda
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  2. Chikako Nishimoto
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  3. Masayuki Miyabe
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  4. Hidenori Toyooka
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Fukuda, T., Nishimoto, C., Miyabe, M. et al. Unilateral adrenalectomy attenuates hemorrhagic shock-induced analgesia in rats. J Anesth 21, 348–353 (2007). https://doi.org/10.1007/s00540-007-0521-2

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  • DOI: https://doi.org/10.1007/s00540-007-0521-2

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