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Modulatory effects of black tea polyphenols on oxidant–antioxidant profile and expression of proliferation, apoptosis, and angiogenesis-associated proteins in the rat forestomach carcinogenesis model

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Abstract

Background

Chemoprevention by dietary constituents has emerged as a novel approach to control stomach cancer incidence. We therefore evaluated the chemopreventive effects of black tea polyphenols (Polyphenon-B) on oxidant–antioxidant status, cell proliferation, apoptosis, and angiogenesis during N-methyl-N′-nitro-N-nitrosoguanidine (MNNG)-induced gastric carcinogenesis.

Methods

Male Wistar rats were divided into four groups. Rats in group 1 and 2 were given MNNG (150 mg/kg body weight) by intragastric intubation three times at 2 week intervals and followed for 26 weeks. Rats in group 2 received in addition a basal diet containing 0.05% Polyphenon-B. Group 3 animals were given 0.05% Polyphenon-B alone. Group 4 animals served as controls. The status of lipid peroxidation and antioxidants and the expression of the lipid peroxidation marker 4-hydroxy nonenal (4-HNE), proliferating cell nuclear antigen (PCNA), glutathiones-transferase (GST)-π, Bcl-2, Bax, cytochrome C, caspase 3, cytokeratins, and vascular endothelial growth factor (VEGF) were used as biomarkers.

Results

Intragastric administration of MNNG induced well-differentiated squamous cell carcinomas that showed diminished lipid and protein oxidation and an increase in antioxidant status. This was associated with increased cell proliferation, angiogenesis, and invasive potential coupled with apoptosis evasion as revealed by upregulation of PCNA, GST-π, Bcl-2, cytokeratins, and VEGF and downregulation of Bax, cytochrome C, and caspase 3 protein expression. Dietary administration of Polyphenon-B effectively suppressed MNNG-induced gastric carcinogenesis, as evidenced by modulation of oxidant–antioxidant status, inhibition of cell proliferation and infiltration, and angiogenesis associated with apoptosis induction.

Conclusions

The present study provides evidence that Polyphenon-B exerts multifunctional inhibitory effects on MNNG-induced gastric carcinogenesis and suggests that it can be developed as a potential chemopreventive agent.

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Authors and Affiliations

  1. Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, 608 002, Tamil Nadu, India

    Ramalingam Senthil Murugan, Kurapathy Venkata Poorna Chandra Mohan & Siddavaram Nagini

  2. Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan

    Koji Uchida

  3. Mitsui Novin Co., Ltd., Tokyo, Japan

    Yukihiko Hara

  4. Department of Pathology, Sri Ramachandra Medical College and Research Institute, Chennai, India

    Duvuru Prathiba

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  1. Ramalingam Senthil Murugan
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  2. Kurapathy Venkata Poorna Chandra Mohan
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  3. Koji Uchida
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  5. Duvuru Prathiba
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  6. Siddavaram Nagini
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Murugan, R., Mohan, K., Uchida, K. et al. Modulatory effects of black tea polyphenols on oxidant–antioxidant profile and expression of proliferation, apoptosis, and angiogenesis-associated proteins in the rat forestomach carcinogenesis model. J Gastroenterol 42, 352–361 (2007). https://doi.org/10.1007/s00535-007-2018-z

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