Zusammenfassung
Die Möglichkeit der in vitro Kurzzeitkultur, d.h. des Wachstums der asexuellen Parasiten vom Trophozoiten zum Schizonten ermöglicht im Prinzip auch für Plasmodium vivax die Entwicklung von Prüfmethoden zur Ermittlung der Arzneimittelempfindlichkeit. Da bei P. vivax keine Sequestration der mit fortgeschrittenen Wachstumsformen infizierten Erythrozyten stattfindet, befinden sich häufig Parasiten aller Wachstumsstadien im peripheren Blut der Patienten. Dies schließt Schizontenreifungstests aus, da deren Ergebnis durch die Anzahl fortgeschrittener Trophozoiten ungleich beeinflusst wird. Ein Testsystem, welches die Altersverteilung der Parasitenpopulation und deren Änderung mit und ohne den Einfluss von Hemmsubstanzen erfassen lässt hat sich bewährt. Es liefert präzise Ergebnisse auch im Bereich der höheren IC Stufen. Dieses Verfahren erlaubt auch die Klarstellung Stadien-spezifischer Aktivität von Malariamitteln.
Summary
The possibility of short-term in vitro cultivation, i.e. growth of asexual erythrocytic stages up to the stage of mature schizonts, permits in principle the development of drug sensitivity tests also for Plasmodium vivax. In the absence of a sequestration of erythrocytes carrying the advanced stages of schizogony, asexual parasites of all stages may be seen in the peripheral blood of patients infected with P. vivax. This precludes schizont maturation tests since schizont development will be unduly influenced by the number of advanced trophozoites. A test system reflecting the age composition of the parasite population and its progression without and under the influence of inhibitors was found to yield precise results also in the higher IC ranges. The population-based test procedure would also permit the identification of any stage-specific impact of antimalarial agents.
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Wernsdorfer, W., Tasanor, O. & Wernsdorfer, G. In vitro drug sensitivity testing in Plasmodium vivax . Wien Klin Wochenschr 120 (Suppl 4), 30–33 (2008). https://doi.org/10.1007/s00508-008-1030-5
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DOI: https://doi.org/10.1007/s00508-008-1030-5