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Using proteomics to study sexual reproduction in angiosperms

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Abstract

While a relative latecomer to the postgenomics era of functional biology, the application of mass spectrometry-based proteomic analysis has increased exponentially over the past 10 years. Some of this increase is the result of transition of chemists, physicists, and mathematicians to the study of biology, and some is due to improved methods, increased instrument sensitivity, and better techniques of bioinformatics-based data analysis. Proteomic Biological processes are typically studied in isolation, and seldom are efforts made to coordinate results obtained using structural, biochemical, and molecular-genetic strategies. Mass spectrometry-based proteomic analysis can serve as a platform to bridge these disparate results and to additionally incorporate both temporal and anatomical considerations. Recently, proteomic analyses have transcended their initial purely descriptive applications and are being employed extensively in studies of posttranslational protein modifications, protein interactions, and control of metabolic networks. Herein, we provide a brief introduction to sample preparation, comparison of gel-based versus gel-free methods, and explanation of data analysis emphasizing plant reproductive applications. We critically review the results from the relatively small number of extant proteomics-based analyses of angiosperm reproduction, from flowers to seedlings, and speculate on the utility of this strategy for future developments and directions.

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Abbreviations

CM:

Central metabolism

CS:

Cell structure

1-DE:

One-dimensional electrophoresis

2-DE:

Two-dimensional electrophoresis

DIGE:

Difference in-gel electrophoresis

GelC-:

A 1-DE variant where after electrophoresis the gel lane is cut into multiple slices

HS:

Hormones and signaling

LC-:

Liquid chromatography

MALDI-:

Matrix-assisted laser-desorption/ionization

MS:

Mass spectrometry

MS/MS:

Tandem MS

MudPIT:

Multi-dimensional protein identification technology

MT:

Membrane transport

NA:

Nucleic acid metabolism

PF:

Protein folding

PMF:

Peptide mass fingerprint

PS:

Protein synthesis

PT:

Protein targeting

PTM:

Posttranslational modifications

PUF:

Proteins of unknown function

SDS–PAGE:

Sodium dodecyl-sulfate polyacrylamide gel electrophoresis

SR:

Stress response

SSP:

Seed storage protein

TOF:

Time of flight

TOF/TOF:

In tandem MS, the first mass analyzer determines the TOF for the precursor (parent) ions then selected ions are diverted to a collision cell fragmented, and the second TOF mass analyzer determines masses of the fragmented ions

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Acknowledgments

This investigation was supported by Seventh Framework Program of the European Union—International Reintegration Grant (MIRG-CT-2007-200165), COST Action FAO903, Bilateral project CSIC-SAS (2007 SK 0001), Project APVV-0115-07, VEGA 2/0005/08 (Study of the cell events in course of embryo formation in situ and in vitro conditions in Arabidopsis and maize) and is a joint publication within the action COST FA 0903, “Harnessing of Plant Reproduction for Crop improvement,” a bilateral Spanish-Slovak cooperation (2007–2009) and the Spanish MEC Project (BFU2006-09876/BFI). Support for JAM was in part from the National Scholarship Program of the Slovak Republic, administered by the Slovak Academic Information Agency. The authors thank B.A. McClure, J.J. Thelen, and two anonymous reviewers for their constructive comments.

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  1. Anna Preťová

    Present address: Department of Botany and Genetics, Faculty of Natural Sciences, The University of Constantine the Philosopher, Trieda A. Hlinku, Nitra, Slovak Republic

Authors and Affiliations

  1. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademicka 2, P. O. Box 39A, 950 07, Nitra, Slovak Republic

    Ján A. Miernyk, Anna Preťová, Katarína Klubicová, Bohuš Obert & Martin Hajduch

  2. USDA, Agricultural Research Service, Plant Genetics Research Unit, Department of Biochemistry, Interdisciplinary Plant Group, University of Missouri, Columbia, MO, 65211, USA

    Ján A. Miernyk

  3. Department of Plant Biochemistry, Cell and Molecular Biology, Estación Experimental de Zaidín (C.S.I.C.), EEZ, Granada, Spain

    Adela Olmedilla

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  1. Ján A. Miernyk
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Correspondence to Martin Hajduch.

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Communicated by Scott Russell.

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Miernyk, J.A., Preťová, A., Olmedilla, A. et al. Using proteomics to study sexual reproduction in angiosperms. Sex Plant Reprod 24, 9–22 (2011). https://doi.org/10.1007/s00497-010-0149-5

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