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High-temperature induction of male sterility during barley (Hordeum vulgare L.) anther development is mediated by transcriptional inhibition

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Abstract

High-temperature induction of male sterility during floral organogenesis is a critical problem for barley (Hordeum vulgare L.) crops that the molecular basis is incompletely understood. Gene expression and differentiation of anthers were examined under normal (20°C day/15°C night) and elevated (30°C day/25°C night) temperatures. Serial analysis of gene expression analysis displayed contrasting profiles of gene expression in early panicles between control and high-temperature conditions. Several transcripts dramatically upregulated before development and differentiation of anther wall layers in normal temperatures, including histone H3, H4 and glycine-rich RNA-binding protein genes, were not upregulated at elevated temperatures and typically abundant mRNAs, such as 60S ribosomal protein L27a and glyoxalase I, appeared to be downregulated. Instead, development and differentiation of tapetum cells and pollen mother cells were completely aborted. Failure of transcriptional reactivation with return to normal temperatures increases with duration of elevated temperatures and is strongly correlated with observation of male sterility. Hyper-phosphorylation of the ser-5 residue of the C-terminal domain of the largest subunit of RNA polymerase II (RPB1) was noted to occur under high-temperature conditions. These results indicate that early development and differentiation of barley anthers are very sensitive to high-temperature stress causing major alterations in gene expression.

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Acknowledgments

We are very grateful to Dr. Tadashi Sato for helpful suggestions and discussions. This work was supported by the Yamada Science Foundation, and by Grant-in Aid (13460113, 16380166) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

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Author notes

  1. Mafumi Abiko

    Present address: Department of Biological Science, The University of Tokyo, Hongo, 113-0033, Japan

Authors and Affiliations

  1. Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Sendai, 980-8577, Japan

    Mafumi Abiko, Kenichi Akibayashi, Tadashi Sakata, Hideyuki Takahashi & Atsushi Higashitani

  2. RIKEN, Bioresource Center, Tsukuba, 305-0074, Japan

    Makoto Kimura

  3. Bioresource Research and Development Laboratories, Sapporo Breweries Ltd., Gunma, 370-0393, Japan

    Makoto Kihara & Kazutoshi Itoh

  4. Kazusa DNA Research Institute, 1532-3, Yana, Kisarazu, 292-0812, Japan

    Erika Asamizu & Shusei Sato

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  1. Mafumi Abiko
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  2. Kenichi Akibayashi
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  4. Makoto Kimura
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  10. Atsushi Higashitani
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Correspondence to Atsushi Higashitani.

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Abiko, M., Akibayashi, K., Sakata, T. et al. High-temperature induction of male sterility during barley (Hordeum vulgare L.) anther development is mediated by transcriptional inhibition. Sex Plant Reprod 18, 91–100 (2005). https://doi.org/10.1007/s00497-005-0004-2

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  • DOI: https://doi.org/10.1007/s00497-005-0004-2

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