Abstract
Hybridisation between certain willow species is a common feature leading to novel genotypes varying in growth rate and stress tolerance. The objective of this 4-week study was to investigate the effects of decreased watering, enhanced ultraviolet-B irradiation (UV-BBE, 280–315 nm, 7.2 kJ m−2 day−1) and combined decreased watering and enhanced UV-B irradiation on di- and polyamines in the leaves of Salix myrsinifolia and its hybrid with S. myrsinites. Control plantlets were well-watered and exposed to ambient UV-B irradiation (UV-BBE, 3.6 kJ m−2 day−1). HPLC analyses showed that the constitutive concentrations of soluble di- and polyamines varied markedly between S. myrsinifolia and its hybrids. The degree of responses to treatments also varied: in S. myrsinifolia, concentrations of free putrescine were clearly increased by reduced watering, while in the hybrid willow, change in putrescine was less pronounced and not significant. Results also showed that the increase in putrescine in S. myrsinifolia by reduced watering was mitigated by concurrent enhancement of UV-B irradiation. There were no direct UV-B effects on the soluble polyamines.
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Acknowledgements
This work was supported by the Academy of Finland (project no. 76681). We also thank Sinikka Sorsa and Maija Makkonen for their help with the micropropagations, and Hanni Sikanen, Mari Tuomainen and Keiko Yamaji for their assistance during the experiment in Punkaharju.
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Tegelberg, R., Turtola, S., Rousi, M. et al. Soluble polyamines in Salix myrsinifolia and S. myrsinites × S. myrsinifolia plantlets exposed to increased UV-B irradiation and decreased watering. Trees 20, 299–303 (2006). https://doi.org/10.1007/s00468-005-0036-0
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DOI: https://doi.org/10.1007/s00468-005-0036-0