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Interleukin-6 and interleukin-8 levels in the urine of children with renal scarring

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Abstract

Background

Acute pyelonephritis (APN) is one of the most significant bacterial infections in infancy and early childhood, and can lead to permanent kidney damage and chronic renal failure.

Objective

To evaluate interleukin-6 (IL-6) and interleukin-8 (IL-8) levels in the urine of children with renal scarring (RS), searching for clinical information about the immuno-inflammatory process that contributes to RS.

Methods

Urine concentrations of IL-6 and IL-8 were evaluated in 50 children, 33 with RS detected after an episode of acute pyelonephritis (group A) and 17 children with a history of acute pyelonephritis, but without RS (group B). These children were divided into four groups: groupA1, 23 children with RS and vesicoureteral reflux (VUR); groupA2, 10 children with RS without VUR; group B1, 13 children without RS and without VUR; group B2, 4 children without RS, but with VUR. None of them had had urinary tract infection for a minimum of 6 months. To avoid dilution effects, urinary levels of IL-6 and IL-8 were expressed as the ratio of cytokine to urinary creatinine (pg/mg).

Results

Urinary IL-8 levels were below the lower detection limit in all samples. IL-6 was detectable in the majority of children with RS and below the detection limits in the urine samples of children without RS. There were no statistically significant differences between urinary interleukin-6 levels in children with and those without VUR. There was a significant relationship between the grade of renal scars, the time passed since the last episode of acute pyelonephritis and the urinary levels of IL-6 (p < 0.0001 and p < 0.04 respectively).

Conclusion

Further experimental studies are required to demonstrate the correlation between histopathology and urinary cytokine levels.

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References

  1. Marild S, Jodal U (1998) Incidence rate of first-time symptomatic urinary tract infection in children under 6 years of age. Acta Paediatr 87:549–552

    Article  PubMed  CAS  Google Scholar 

  2. Jacobson SH, Eklof O, Eikson CG, Lins LE, Tidgren B, Winberg J (1989) Development of hypertension and uraemia after pyelonephritis in childhood: 27 years follow-up. BMJ 299(6701):703–706

    Article  PubMed  CAS  Google Scholar 

  3. Jacobson SH, Eklof O, Lins LE, Wikstad I, Winberg J (1992) Long term prognosis of post infectious renal scarring in relation to radiological findings in childhood: 27 year follow-up. Pediatr Nephrol 6:19–24

    Article  PubMed  CAS  Google Scholar 

  4. Glauser MP, Meylan P, Bille J (1987) The inflammatory response and tissue damage. Pediatr Nephrol 1(4):615–622

    Article  PubMed  CAS  Google Scholar 

  5. Otto G, Branconier J, Andreasson A, Svanborg C (1999) Interleukin-6 and disease severity in patients with bacteremic and nonbacteremic febrile urinary tract infection. J Infect Dis 179:172–179

    Article  PubMed  CAS  Google Scholar 

  6. Haraoka M, Matsumoto T, Takahashi K, Kubo S, Tanaka M, Kumazawa J (1994) Suppression of renal scarring by prednisolone combined with ciprofloxacin in ascending pyelonephritis in rats. J Urol 151:1078–1085

    PubMed  CAS  Google Scholar 

  7. Risdon RA (1994) Renal pathology in 99mTc-DMSA image during the evolution of the early pyelonephritic scar: an experimental study. J Urol 151:767–773

    PubMed  CAS  Google Scholar 

  8. Tullus K, Fituri O, Burman LG, Wretlind B, Brauner A (1994) Interleukin-6 and interleukin-8 in the urine of children with acute pyelonephritis. Pediatr Nephrol 8:280–284

    Article  PubMed  CAS  Google Scholar 

  9. Jacobson S, Hylander B, Wretlind B, Brauner A (1994) Interleukin-6 and interleukin-8 in serum and urine in patients with acute pyelonephritis in relation to bacterial-virulence-associated traits and renal function. Nephron 67:172–179

    Article  PubMed  CAS  Google Scholar 

  10. International Reflux Study Committee (1981) Medical versus surgical treatment of primary vesicoureteral reflux: a prospective international reflux study in children. J Urol 125:277–285

    Google Scholar 

  11. Goldraich NP, Goldraich IH (1995) Update on dimercaptosuccinic acid renal scanning in children with urinary tract infection. Pediatr Nephrol 9:221–227

    Article  PubMed  CAS  Google Scholar 

  12. Rushton HG, Majd M, Jantausch B, Wiedermann RI, Belman AB (1992) Renal scarring following reflux and non-reflux pyelonephritis in children: evaluation with 99 m-technitium-dimercapto-succcinic acid scintigraphy. J Urol 147:1327–1332

    PubMed  CAS  Google Scholar 

  13. Cortan RS (1982) Glomerulosclerosis in reflux nephropathy. Kidney Int 21:528–534

    Article  Google Scholar 

  14. Rugo H, O Hanley P, Bishop A, Pearce M, Abrams J, Howard M (1992) Local cytokine production in a murine model of Escherichia coli pyelonephritis. J Clin Invest 89:1032–1039

    Article  PubMed  CAS  Google Scholar 

  15. Roilides E, Papachristou F, Gioulekas E, Tsaparidou S, Karatzas N, Sotiriou J, Tsiouris J (1999) Increased urine interleukin-6 concentrations correlate with pyelonephritic changes on 99mTc-dirmecaptosuccinic acid scans in neonates with urinary tract infections. J Infect Dis 180:904–907

    Article  PubMed  CAS  Google Scholar 

  16. Schmouder R, Strieter R, Wiggins R, Chensue S, Kunkel S (1992) In vitro and in vivo intereukin-8 production in human renal cortical epithelial. Kidney Int 41:191–198

    Article  PubMed  CAS  Google Scholar 

  17. Haraoka M, Senoh K, Ogata N, Furukawa M, Matsumoto T, Kumazawa J (1996) Elevated interleukin-8 levels in the urine of children with renal scarring and/or vesicoureteral reflux. J Urol 155:678–680

    Article  PubMed  CAS  Google Scholar 

  18. Gokce I, Alpay H, Biyikli N, Unluguzel G, Dede F, Topuzoglu A (2010) Urinary levels of interleukin-6 and interleukin-8 in patients with vesicoureteral reflux and renal parenchymal scar. Pediatr Nephrol 5:905–912

    Article  Google Scholar 

  19. Galanakis E, Bitsori M, Dimitriou H, Giannakopoulou C, Karkavitsas NS, Kalmanti M (2006) Urine interleukin-8 as a marker of vesicoureteral reflux in infants. Pediatrics 117:e863–e867

    Article  PubMed  Google Scholar 

  20. Anders HJ, Banas B, Schlondorff D (2004) Signaling danger: toll-like receptors and their potential roles in kidney disease. J Am Soc Nephrol 15:854–867

    Article  PubMed  CAS  Google Scholar 

  21. Wolfs TG, Buurman WA, van Schadewijk A, de Vries B, Daemen MA, Hiemstra PS, van't Veer C (2002) In vivo expression of Toll-like receptor 2 and 4 by renal epithelial cells: IFN-gamma and TNF-alpha mediated up-regulation during inflammation. J Immunol 168:1286–1293

    PubMed  CAS  Google Scholar 

  22. Kim BS, Lim SW, Li C, Kim JS, Sun BK, Ahn KO, Han SW, Kim J, Yang CW (2005) Ischemia-reperfusion injury activates innate immunity in rat kidneys. Transplantation 79:1370–1377

    Article  PubMed  Google Scholar 

  23. Song J, Duncan JM, Li G, Chan C, Grady R, Stapleton A, Abraham NS (2007) A novel TLR-4 mediated signaling pathway leading to IL-6 responses in human bladder epithelial cells. PLoS Pathogens 3(4):540–552

    Article  Google Scholar 

  24. Cunningham PN, Wang Y, Guo R, He G, Quigg RJ (2004) Role of Toll-like receptor 4 in endotoxin-induced acute renal failure. J Immunol 172:2629–2635

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. 4th Pediatric Department, Aristotle University of Thessaloniki, Thessaloniki, Greece

    Despoina Tramma & Vasilis Lafazanis

  2. 2nd Pediatric Department, Aristotle University of Thessaloniki, Thessaloniki, Greece

    Maria Hatzistylianou

  3. Nuclear Medicine Department, Aristotle University of Thessaloniki, Thessaloniki, Greece

    George Gerasimou

  4. 4th Pediatric Department, Papageorgiou Hospital, Efcarpia Ring Road, 54636, Thessaloniki, Greece

    Despoina Tramma

Authors
  1. Despoina Tramma
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  2. Maria Hatzistylianou
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  3. George Gerasimou
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  4. Vasilis Lafazanis
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Correspondence to Despoina Tramma.

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Tramma, D., Hatzistylianou, M., Gerasimou, G. et al. Interleukin-6 and interleukin-8 levels in the urine of children with renal scarring. Pediatr Nephrol 27, 1525–1530 (2012). https://doi.org/10.1007/s00467-012-2156-2

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  • DOI: https://doi.org/10.1007/s00467-012-2156-2

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