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Heat shock proteins in children and young adults on chronic hemodialysis

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Abstract

Chronic inflammation, lipid and autoimmune disorders are hallmarks of atherogenesis, and hemodialysis per se may be an additional factor predisposing to accelerated atherosclerosis. Elevated levels of heat shock proteins (HSP) and antibodies against these HSP have been described in adults with atherosclerotic lesions and cardiovascular events, but to date there has been a scarcity of investigations on these parameters in adult and pediatric patients on hemodialysis (HD). We have investigated the HSP profile in hemodialyzed children and the impact of a single HD session on those proteins and their correlations with known risk factors for atherosclerosis. The study group consisted of 17 children and young adults undergoing HD with polysulfone membranes. The control group comprised 15 age-matched subjects with normal kidney function. The serum concentrations of Hsp60, Hsp90alpha, anti-Hsp60, anti-Hsp70, and sE-selectin were assessed by an enzyme-linked immunosorbent assay, and serum concentration of high-sensitivity-C-reactive protein was assayed by nephelometry. The serum lipid profile [total cholesterol (CHOL), high-density lipoprotein-CHOL, low-density lipoprotein-CHOL, triglycerides] was also estimated. Compared to the control values, the median values of Hsp60 before the HD session were lower, whereas those of Hsp90alpha and anti-Hsp60 were higher. A single HD session raised the median values of Hsp60 and Hsp90alpha and decreased the concentrations of anti-Hsp60 and anti-Hsp70. In addition, the concentrations of HSPs and the antibodies against them correlated with the lipid markers both before and after HD. The altered HSP and anti-HSP concentrations in HD children, which correlated with the lipid profile and the endothelial markers, suggest a dysfunctional HSP system in this population and the possibility of HSPs being classified as new markers of atherosclerosis.

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Authors and Affiliations

  1. Department of Pediatric Nephrology, Wrocław Medical University, M.Skłodowskiej–Curie 50/52, 50-369, Wrocław, Poland

    Kinga Musiał & Danuta Zwolińska

  2. Department of Pediatrics, Clinic of Nephrology, Endocrinology and Metabolic Diseases of Childhood, Silesian School of Medicine, ul. 3 Maja 13/15, Zabrze, 41-800, Poland

    Krystyna Szprynger & Maria Szczepańska

Authors
  1. Kinga Musiał
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  2. Krystyna Szprynger
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  3. Maria Szczepańska
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  4. Danuta Zwolińska
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Correspondence to Danuta Zwolińska.

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Musiał, K., Szprynger, K., Szczepańska, M. et al. Heat shock proteins in children and young adults on chronic hemodialysis. Pediatr Nephrol 24, 2029–2034 (2009). https://doi.org/10.1007/s00467-009-1197-7

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  • DOI: https://doi.org/10.1007/s00467-009-1197-7

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