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Teaching molecular genetics: Chapter 3 – Proteomics in nephrology

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Abstract

The novel discipline of proteomics has experienced a rapid growth in the recent past and has great potentials for the future. The study of proteins on a genomic scale enables a large number of proteins to be analysed simultaneously. Moreover, proteomic analysis reveals the presence of protein isoforms and post-translational modifications, both of which have the potential to regulate protein complex formation, activity and function. As such, the assessment of the proteome, unlike genomic analysis, provides a view of biological processes at their level of occurrence. The knowledge thus gained is important not only for a better understanding of renal physiology and pathophysiology, but also for the identification of disease markers and the development of new therapies. This review applies the science of proteomics to nephrology: our aim is to give an overview of the discipline, providing background information and outlining the scope, advantages and limitations of proteomics.

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Acknowledgements

The authors would like to acknowledge Wendy Pluk for her valuable technical assistance with the nanoLC-MS/MS, Joyce Geelen for providing data of the HUVEC proteome study and Bart Smeets and Mark Steenbergen for providing the 2D-gel electrophoresis protein map of mouse glomeruli.

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Authors and Affiliations

  1. Department of Pathology, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands

    Patricia J. T. A. Groenen

  2. Pediatric Nephrology, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands

    Lambert P. W. J. van den Heuvel

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  1. Patricia J. T. A. Groenen
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  2. Lambert P. W. J. van den Heuvel
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Correspondence to Patricia J. T. A. Groenen.

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Groenen, P.J.T.A., van den Heuvel, L.P.W.J. Teaching molecular genetics: Chapter 3 – Proteomics in nephrology. Pediatr Nephrol 21, 611–618 (2006). https://doi.org/10.1007/s00467-006-0064-z

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