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The association between transforming growth factor-β gene promoter C-509T polymorphism and Chinese children with Henoch-Schönlein purpura

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Abstract

Many cytokines, including transforming growth factor-β (TGF-β) and tumor necrosis factor-α (TNF-α), are involved in the inflammatory process of Henoch-Schönlein purpura (HSP). The objective of this study was to investigate whether TGF-β C-509T and TNF-α G-308A polymorphisms are associated with childhood HSP. The loci of interest were amplified from genomic DNA using specific primers and polymerase chain reaction, and these two polymorphisms were compared between Chinese children with HSP and healthy controls. The disease severities evaluated and expressed as symptom score of patients with different genotypes were also compared. The TGF-β -509 TT genotype was more common in children with HSP than controls (31% vs. 8%, P =0.03, odds ratio=4.95). The allelic frequencies of TGF-β -509, genotypic and allelic frequencies of TNF-α -308 were not significantly different. Patients with the TT genotype had more severe clinical presentations than non-TT (TC+CC) patients (4.1±0.42 vs. 2.7±0.31, P =0.018). These results suggest that the TT genotype of the C-509T polymorphism of the TGF-β gene might be related to the susceptibility of Chinese children to HSP and to the severity of this disease.

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References

  1. Cassidy JT, Petty RE (2001) Textbook of pediatric rheumatology, 4th edn. Saunders, Philadelphia, pp 569–574

  2. Lin SJ, Huang JL (1998) Henoch-Schönlein purpura in Chinese children and adults. Asian Pac J Allergy Immunol 16:21–25

    CAS  PubMed  Google Scholar 

  3. Yang YH, Huang MT, Lin SC, Lin YT, Tsai MJ, Chiang BL (2000) Increased TGF-β secreting T cells and IgA anticardiolipin antibodies levels during acute stage of childhood Henoch-Schönlein purpura. Clin Exp Immunol 122:285–290

    Article  CAS  PubMed  Google Scholar 

  4. Yang YH, Wang SJ, Chuang YH, Lin YT, Chiang BL (2002) The level of IgA antibodies to human umbilical vein endothelial cells can be enhanced by TNF-α treatment in children with Henoch-Schönlein purpura. Clin Exp Immunol 130:352–357

    Article  CAS  PubMed  Google Scholar 

  5. Ivanovic V, Melman A, Davis-Joseph B, Valcic M, Geliebter J (1995) Elevated plasma levels of TGF-β1 in patients with invasive prostate cancer. Nat Med 1:282–284

    CAS  PubMed  Google Scholar 

  6. Grainger DJ, Kemp PR, Metcalfe JC, Liu AC, Lawn RM, Williams NR, Grace AA, Schofield PM, Chauhan A (1995) The serum concentration of active transforming growth factor-β is severely depressed in advanced atherosclerosis. Nat Med 1:74–79

    CAS  PubMed  Google Scholar 

  7. Kulkarni AB, Ward JM, Yaswen L, Mackall CL, Bauer SR, Huh CG, Gress RE, Karlsson S (1995) Transforming growth factor-beta1 null mice—an animal model for inflammatory disorders. Am J Pathol 146:264–275

    CAS  PubMed  Google Scholar 

  8. Nalbant S, Koc B, Top C, Kucukardali Y, Baykal Y, Danaci M, Kocer IH (2002) Hypersensitivity vasculitis and cytokines. Rheumatol Int 22:244–248

    Article  CAS  PubMed  Google Scholar 

  9. De Mattia D, Penza R, Giordano P, Del Vecchio GC, Aceto G, Altomare M, Schettini F (1995) von Willebrand factor and factor XIII in children with Henoch-Schönlein purpura. Pediatr Nephrol 9:603–605

    PubMed  Google Scholar 

  10. Grainger DJ, Heathcote K, Chiano M, Snieder H, Kemp PR, Metcalfe JC, Carter ND, Spector TD (1999) Genetic control of circulating concentration of transforming growth factor type β1. Hum Mol Genet 8:93–97

    Article  CAS  PubMed  Google Scholar 

  11. Wilson AG, Giovine FS di, Blakemore AIF, Duff GW (1992) Single base polymorphism in the human tumor necrosis factor alpha (TNFα) gene detectable by Nco 1 restriction of PCR product. Hum Mol Genet 1:353

    CAS  Google Scholar 

  12. Amoli MM, Thomson W, Hajeer AH, Calvino MC, Garcia-Porrua C, Ollier WE, Gonzalez-Gay MA (2001) HLA-DRB1*01 association with Henoch-Schönlein purpura in patients from Northwest Spain. J Rheumatol 28:1266–1270

    CAS  PubMed  Google Scholar 

  13. Amoli MM, Thomson W, Hajeer AH, Calvino MC, Garcia-Porrua C, Ollier WE, Gonzalez-Gay MA (2002) Interleukin-1 receptor antagonist gene polymorphism is associated with severe renal involvement and renal sequelae in Henoch-Schönlein purpura. J Rheumatol 29:1404–1407

    CAS  PubMed  Google Scholar 

  14. Amoli MM, Thomson W, Hajeer AH, Calvino MC, Garcia-Porrua C, Ollier WE, Gonzalez-Gay MA (2002) Interleukin 8 gene polymorphism is associated with increased risk of nephritis in cutaneous vasculitis. J Rheumatol 29:2367–2370

    CAS  PubMed  Google Scholar 

  15. Quasney MW, Bronstein DE, Cantor RM, Zhang Q, Stroupe C, Shike H, Bastian JF, Matsubara T, Fujiwara M, Akimoto K, Newburger JW, Burns JC (2001) Increased frequency of alleles associated with elevated tumor necrosis factor-α levels in children with Kawasaki disease. Pedatr Res 49:686–690

    CAS  Google Scholar 

  16. Albuquerque RV, Hayden CM, Palmer LJ, Laing IA, Rye PJ, Gibson NA, Burton PR, Goldblatt J, Lesouef PN (1998) Association of polymorphisms within the tumour necrosis factor (TNF) genes and childhood asthma. Clin Exp Allergy 28:578–584

    Article  CAS  PubMed  Google Scholar 

  17. Higuchi T, Seki N, Kamizono S, Yamada A, Kimura A, Kato H, Itoh K (1998) Polymorphism of the 5’-flanking region of the human tumor necrosis factor (TNF)-α gene in Japanese. Tissue Antigens 51:605–612

    CAS  PubMed  Google Scholar 

  18. Pociot F, Briant L, Jongeneel CV, Molvig J, Worsaae H, Abbal M, Thomsen M, Nerup J, Cambon-Thomsen A (1993) Association of tumor necrosis factor (TNF) and class II major histocompatibility complex alleles with the secretion of TNF-α and TNF-β by human mononuclear cells: a possible link to insulin-dependent diabetes mellitus. Eur J Immunol 23:224–231

    CAS  PubMed  Google Scholar 

  19. Louis E, Franchimont D, Piron A, Gevaert Y, Schaaf-Lafontaine N, Roland S, Mahieu P, Malaise M, De Groote D, Louis R, Belaiche J (1998) Tumour necrosis factor (TNF) gene polymorphism influences TNF-α production in lipopolysaccharide (LPS)-stimulated whole blood cell culture in healthy humans. Clin Exp Immunol 113:401–406

    Article  CAS  PubMed  Google Scholar 

  20. Waage A, Halstensen A, Espevik T (1987) Association between tumour necrosis factor in serum and fatal outcome in patients with meningococcal disease. Lancet I:355–357

    Article  Google Scholar 

  21. Grau GE, Taylor TE, Molyneux ME, Wirima JJ, Vassalli P, Hommel M, Lambert PH (1989) Tumor necrosis factor and disease severity in children with falciparum malaria. N Engl J Med 320:1586–1591

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan

    Yao-Hsu Yang, Huey-Jen Lai, Cheng-Kai Kao, Yu-Tsan Lin & Bor-Luen Chiang

  2. Department of Pediatrics, National Taiwan University Hospital, No. 7 Chung-Shan South Road, Taipei, Taiwan

    Bor-Luen Chiang

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  1. Yao-Hsu Yang
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  2. Huey-Jen Lai
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Correspondence to Bor-Luen Chiang.

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Yang, YH., Lai, HJ., Kao, CK. et al. The association between transforming growth factor-β gene promoter C-509T polymorphism and Chinese children with Henoch-Schönlein purpura. Pediatr Nephrol 19, 972–975 (2004). https://doi.org/10.1007/s00467-004-1547-4

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  • DOI: https://doi.org/10.1007/s00467-004-1547-4

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