Abstract
Background
CO2 pneumoperitoneum provides a new surgical environment to treat malignant disease. The purpose of this study was to investigate the influence of CO2 pneumoperitoneum during laparoscopic surgery on cancer cell growth.
Methods
WiDr human colon cancer cells were incubated for 3 h under the following two conditions: 100% CO2 at 10 mmHg, and 95% air/5% CO2 (control). Cell proliferation was assessed by the WST-1 assay and BrdU assay. Tumor growth was assessed by subcutaneous injection into 20 nude mice. Cellular damage was measured by lactate dehydrogenase (LDH) assay.
Results
The number of WiDr cells under pneumoperitoneal conditions decreased in the first 24 h. However, no significant difference was observed in the proliferation rate and tumor growth of the viable cells. LDH release of the CO2 pneumoperitoneal group was higher than that of the controls.
Conclusions
Our data indicate that CO2 pneumoperitoneum does not promote cancer cell proliferation but instead has a toxic effect on cancer cells.
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References
Allendorf JDF, Bessler M, Horvath KD, Marvin MR, Laird DA, Whelan RL (1998) Increased tumor establishment and growth after open vs laparoscopic bowel resection in mice. Surg Endosc 12: 1035–1038
Baguchi D, Baguchi M, Hassoun EA, Stohs SJ (1995) In vitro and in vivo generation of reactive oxygen species, DNA damage and lactate dehydrogenase leakage by selected pesticides. Toxicology 104: 129–140
Berman IR (1995) Laparoscopic colectomy for cancer: some cause for pause. Ann Surg Oncol 2: 1–2
Bouvy ND, Marquet RL, Hamming JF, Jeekel J, Bonjer HJ (1996) Laparoscopic surgery in the rat: beneficial effect on body weight and tumor take. Surg Endosc 10: 490–494
Bouvy ND, Marquet RL, Jeekel J, Bonjer HJ (1997) Laparoscopic surgery is associated with less tumor growth stimulation than conventional surgery: an experimental study. Br J Surg 84: 358–361
Collet D, Vitale GC, Reynolds M, Klar E, Cheadle WG (1995) Peritoneal host defenses are less impaired by laparoscopy than by open operation. Surg Endosc 9: 1059–1064
Fielding GA, Lumley J, Nathanson L, Hewitt P, Rhodes M, Stitz R (1997) Laparoscopic colon surgery. Surg Endosc 11: 745–749
Gutt CN, Heinz P, Kaps W, Paolucci V (1997) The phagocytosis activity during conventional surgery and laparoscopic operations in the rat. Surg Endosc 11: 899–901
Hall GM, Desborough JP (1992) Interleukin-6 and metabolic response to surgery. Br J Anaesth 69: 337–338
Horgan PG, Fitzpatrick M, Couse NF, Gorey TF, Fitzpatrick JM (1992) Laparoscopy is less immunotraumatic than laparotomy. Min Inv Ther 1: 241–244
Jacobi CA, Ordemann J, Böhem B, Zieren HU, Liebenthal C, Volk HD, Müller JM (1997) The Influence of laparotomy and laparoscopy on tumor growth in a rat model. Surg Endosc 11: 618–621
Jones DB, Guo LW, Reinhard MK, Soper MJ, Philpott GW, Conett J, Fleshman JM (1995) Impact of pneumoperitoneum on trocar site implantation of colon cancer in hamster model. Dis Colon Rectum 38: 1182–1188
Köckerling F, Schneider C, Reymond MA, Scheidbach H, Konradt J, Bärlehner E, Bruch HP, Kuthe A, Troidl H, Hohenberger W (1998) Early results of a prospective multicenter study on 500 consecutive cases of laparoscopic colorectal surgery. Surg Endosc 12: 37–41
Lacy AM, Delgado S, Galcia-Valdecass JC, Castells A, Piqué JM, Grande L, Fuster J, Targarona EM, Pera M, Visa J (1998) Port site metastases and recurrence after laparoscopic colectomy: A randomized trial. Surg Endosc 12: 1039–1042
Mathew G, Watson DI, Rofe AM, Baigrie CF, Ellis T, Jamieson GG (1996) Wound metastasis following laparoscopic and open abdominal surgery for abdominal cancer in a rat model. Br J Surg 83: 1087–1090
Roumen RM, Van-meurs PA, Kuypers HH, Kraak WA, Sauerwein RW (1992) Serum interleukin-6 and C reactive protein responses in patients after laparoscopic or conventional cholecystectomy. Eur J Surg 158: 541–544
Trokel MJ, Allendorf JDF, Treat MR, Whelan RL, Nowygrod R, Bessler M (1994) Inflammatory response in better preserved after laparoscopy vs. laparotomy. Surg Endosc 8: 232
West MA, Hackman DJ, Baker J, Rodriguez JL, Bellingham J, Rotstein OD (1997) Mechanism of decreased in vitro murine macrophage cytokine release after exposure to carbon dioxide. Ann Surg 226: 179–190
Wexner SD, Cohen SM (1995) Port site metastases after laparoscopic colorectal surgery for cure of malignancy. Br J Surg 82: 295–298
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Takiguchi, S., Matsuura, N., Hamada, Y. et al. Influence of CO2 pneumoperitoneum during laparoscopic surgery on cancer cell growth. Surg Endosc 14, 41–44 (2000). https://doi.org/10.1007/s004649900008
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DOI: https://doi.org/10.1007/s004649900008