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Toll-like receptors in the inflammatory response during open and laparoscopic colectomy for colorectal cancer

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Abstract

Background

Surgical interventions activate a cascade of reactions that result in an aseptic inflammatory reaction. This inflammatory response initiates the organism’s innate immunity. Laparoscopic surgery reduces the trauma, and patients benefit from diminished surgical trauma and maintained immune function. Cytokine levels and C-reactive protein (CRP) are related to the magnitude of surgical trauma and surgical stress. Toll-like receptors (TLRs) 2 and 4 are the first sensor-recognition receptors of the invading pathogens for the innate immune response. This study aimed to compare the inflammatory response and then the stress response during laparoscopic and open colectomy for cancer by calculating TLR-2 and TLR-4 as the first sensor-recognition receptors together with interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and high-sensitivity CRP (hsCRP).

Methods

A total 40 patients with colorectal cancer were randomized in two groups: group A (open colectomy, n = 20) and group B (laparoscopic colectomy, n = 20). An epidural catheter was placed in all patients 1 h preoperatively. Rupivocaine was administered perioperatively and 48 h postoperatively. Blood samples were taken for calculation of IL-6, TNF-α, hsCRP, TLR-2, and TLR-4 preoperatively and 5 min after deflation of pneumoperitoneum (group B) or 5 min after division of the colon (group A), then 6 and 24 h postoperatively.

Results

The mean operative time was 115 for group A and 142 min for group B. The mean blood loss was respectively 240 and 105 ml (P < 0.001), and the mean hospital stay was respectively 8 and 5 days (P < 0.05). The IL-6 level was significant higher in group A than in group B at 6 and 24 h postoperatively (P < 0.0001), and the hsCRP level was significant higher in group A than in group B at 24 h postoperatively (P < 0.001). The TNF-α values did not differ between the two groups. The TLR-2 level was significantly higher in group A than in group B at 5 min (P = 0.013) and 24 h (P = 0.007) postoperatively. The TLR-4 level was significant higher in group A than in group B at 5 min postoperatively (P = 0.03).

Conclusion

The inflammatory response and the resultant stress response are significantly less during laparoscopic colectomy than during open colectomy for colorectal cancer. This is an obvious short-term clinical benefit for the patient, providing tinder for further study to investigate the long-term results of laparoscopic colectomy versus open colectomy for colorectal cancer (Registered clinical trial no. NCT00942461; www.clinicaltrials.gov).

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Acknowledgments

The authors thank Professor Charalampos Moutsopoulos, MD, PhD, Chairman of Pathologic Physiology at Athens University, School of Medicine, for the help with knowledge concerning TLRs.

Disclosures

Konstantinos E. Tsimogiannis, Constantinos C. Tellis, Alexandros D. Tselepis, George K. Pappas-Gogos, Evangelos C. Tsimoyiannis, and George Basdanis have no conflicts of interest or financial ties to disclose.

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Authors and Affiliations

  1. Department of Surgery, “G. Hatzikosta” General Hospital, Hippocratus 3, Stavraki, GR-45332, Ioannina, Greece

    Konstantinos E. Tsimogiannis, George K. Pappas-Gogos & Evangelos C. Tsimoyiannis

  2. Laboratory of Biochemistry, Chemistry Department, Ioannina University, Ioannina, Greece

    Constantinos C. Tellis & Alexandros D. Tselepis

  3. First Surgical Department, Aristotle University, Thessaloniki, Greece

    George Basdanis

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  1. Konstantinos E. Tsimogiannis
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  2. Constantinos C. Tellis
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Correspondence to Konstantinos E. Tsimogiannis.

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Tsimogiannis, K.E., Tellis, C.C., Tselepis, A.D. et al. Toll-like receptors in the inflammatory response during open and laparoscopic colectomy for colorectal cancer. Surg Endosc 26, 330–336 (2012). https://doi.org/10.1007/s00464-011-1871-2

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  • DOI: https://doi.org/10.1007/s00464-011-1871-2

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