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Pushing the envelope in biomaterial research: initial results of prosthetic coating with stem cells in a rat model

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Abstract

Background

Coating prosthetic for hernia repair with a patient’s own cells could improve biocompatibility by decreasing inflammation and adhesion formation and by increasing tissue ingrowth and resistance to infection. The objective of this study was to prove the feasibility of prosthetic coating with stem cells and to assess its resistance to adhesion formation when implanted in an animal model.

Methods

Adult Lewis rat bone marrow stem cells were harvested and cultured. Stem cells were then implanted on three different prosthetics. The prosthetic with the best stem cell adherence was implanted intraperitoneally into six adult rats. Untreated prosthetic was implanted in control animals (n = 12). After 2 weeks, intra-abdominal adhesions were graded using an adhesion scoring scale by two surgeons who were blinded to the animal group. Data were analyzed using the Wilcoxon rank-sum test.

Results

Stem cells demonstrated the best adherence and growth on polyglactin prosthetics. After implantation, the stem cell-coated polyglactin prosthetic had <25% of its surface area covered with adhesions in five (83%) samples, whereas the control polyglactin group had only one sample (8.3%) with <25% adhesions, and seven of its samples (58.3%) had >50% surface area adhesions (p < 0.05).

Conclusions

The feasibility of hernia prosthetic coating with stem cells was demonstrated. Furthermore, stem-cell coated polyglactin prosthetic exhibited improved biocompatibility by decreasing adhesion formation in an animal model. Further study is needed to determine the factors that promote stem cell adherence to prosthetics and the in vivo prosthetic biomechanics after stem cell coating. This work is underway in our laboratory.

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Acknowledgments

This study was in part funded by the Society of American Gastrointestinal and Endoscopic Surgeons.

Disclosures

Drs. Charles J. Dolce, Dimitrios Stefanidis, Jennifer E. Keller, K. Christian Walters, William L. Newcomb, H. James Norton, Amy E. Lincourt, Kent W. Kercher, B. Todd Heniford, and Jessica J. Heath, have no conflicts of interest or financial ties to disclose.

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Authors and Affiliations

  1. Department of Gastrointestinal and Minimally Invasive Surgery, Department of Surgery, Carolinas Medical Center, 1000 Blythe Blvd. MEB#601, Charlotte, NC, 28203, USA

    Charles J. Dolce, Dimitrios Stefanidis, Jennifer E. Keller, K. C. Walters, William L. Newcomb, Jessica J. Heath, H. J. Norton, Amy E. Lincourt, Kent W. Kercher & B. T. Heniford

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  1. Charles J. Dolce
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  2. Dimitrios Stefanidis
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  3. Jennifer E. Keller
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  4. K. C. Walters
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  5. William L. Newcomb
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  6. Jessica J. Heath
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  7. H. J. Norton
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  8. Amy E. Lincourt
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  9. Kent W. Kercher
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  10. B. T. Heniford
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Corresponding author

Correspondence to B. T. Heniford.

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Dolce, C.J., Stefanidis, D., Keller, J.E. et al. Pushing the envelope in biomaterial research: initial results of prosthetic coating with stem cells in a rat model. Surg Endosc 24, 2687–2693 (2010). https://doi.org/10.1007/s00464-010-1026-x

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  • DOI: https://doi.org/10.1007/s00464-010-1026-x

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