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Laparoscopic bowel injury in an animal model: monocyte migration and apoptosis

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Abstract

Background

Unrecognized laparoscopic bowel injury has a delayed and covert presentation. Differences in monocyte migration and apoptosis between laparoscopic and open bowel injury were determined.

Methods

For this study, 24 rabbits were divided into laparoscopic (n = 9) and open surgical (n = 9) bowel injury groups and a control group (n = 6) without bowel injury. Bowel injury was created using monopolar electrocautery. The animals were killed 1 day, 1 week, and 2 weeks after surgery. Monocyte migration assay was performed across a modified Boyden chamber. Apoptosis was assessed by DNA fluorescent stain H-33342.

Results

In laparoscopy, monocyte apoptosis was decreased (p < 0.001), and migration was increased (p < 0.05), as compared with the open group. Apoptosis increased over time in both study groups, and was higher than in the control group (p < 0.001). Migration was decreased in both study groups as compared with the control group (p < 0.05)

Conclusions

These results suggest decreased immune system priming with laparoscopic bowel injury, which may contribute to the masking of relevant signs and symptoms of peritonitis.

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Acknowledgment

This work was supported by a grant from the New York Academy of Medicine, The Ferdinand C. Valentine Award.

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Authors and Affiliations

  1. Department of Urology, Long Island Jewish Medical Center, 270-05 76th Avenue, New Hyde Park, NY, 11040-1496, USA

    A. El-Hakim, J. P. A. Aldana & B. R. Lee

  2. Department of Nephrology, Long Island Jewish Medical Center, 270-05 76th avenue, New Hyde Park, NY , 11040-1496, USA

    K. Reddy & P. Singhal

Authors
  1. A. El-Hakim
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  2. J. P. A. Aldana
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  3. K. Reddy
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  4. P. Singhal
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  5. B. R. Lee
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El-Hakim, A., Aldana, J.P., Reddy, K. et al. Laparoscopic bowel injury in an animal model: monocyte migration and apoptosis. Surg Endosc 19, 484–487 (2005). https://doi.org/10.1007/s00464-004-8152-2

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  • DOI: https://doi.org/10.1007/s00464-004-8152-2

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