Skip to main content

Advertisement

SpringerLink
Log in

Impact of taurolidine on the growth of CC531 coloncarcinoma cells in vitro and in a laparoscopic animal model in rats

  • Original article
  • Published:
Surgical Endoscopy And Other Interventional Techniques Aims and scope Submit manuscript

Abstract

Background

The object of this study was to examine the effect of taurolidine on intraabdominal tumor growth in a laparoscopic animal model. We tested the cytotoxic, antiadhesive, and anti-invasive effects of this substance on CC531 adenocarcinoma cells in vitro and in vivo using WAG rats.

Methods

For in vitro experiments, Transwell dual chambers with polycarbonate filters coated with 100 μg/cm2 Matrigel were used to investigate the effects of 5, 10, and 20 μl of 2.0% taurolidine on the invasion of 1 × 105 CC531 adenocarcinoma cells. For the adhesion assays, tumor cells were applied onto microtiter plates coated with 5, 10, and 20 μl taurolidine and 0.9% NaCl solution for the control group subsequently. For in vivo experiments, 40 WAG rats were randomized into three therapy groups and one control group. All animals underwent laparoscopy and received 1 ml of CC531 adenocarcinoma cells (5 × 106 cells/ml) intraabdominally at the beginning of the procedure. According to the randomization, the rats were administered taurolidine with different concentrations or 1 ml of 0.9% NaCl solution for the control group. After 21 days, the animals were killed and the intraabdominal tumor weight was determined.

Results

For the in vitro experiments, we found a moderate cytotoxicity and a significant inhibition of tumor cell adhesion and invasion (p < 0.01) by all taurolidine concentrations used in the assay. For in vivo experiments, the application of all concentrations of taurolidine significantly decreased the intraperitoneal tumor weight (p < 0.001).

Conclusion

Taurolidine significantly decreases adhesion and invasion of CC531 adenocarcinoma cells in vitro and significantly diminishes tumor growth in vivo. This may offer additional therapeutic options for laparoscopic surgery for colorectal cancer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1
Figure 2
Figure 3

Similar content being viewed by others

References

  1. JD Allendorf M Bessler KD Horvath MR Marvin DA Laird RL Whelan (1999) ArticleTitleIncreased tumor establishment and growth after open vs laparoscopic surgery in mice may be related to differences in postoperative T-cell function Surg Endosc 13 233–235 Occurrence Handle1:STN:280:DyaK1M7msF2jsg%3D%3D Occurrence Handle10064753

    CAS  PubMed  Google Scholar 

  2. R Bieselt (1997) ArticleTitleSurgical therapy of pleural empyema with tauroline Langenbecks Arch Chir 382 S42–S46 Occurrence Handle1:STN:280:ByiH3M7ltlU%3D Occurrence Handle9333707

    CAS  PubMed  Google Scholar 

  3. JI Blenkharh (1988) ArticleTitleSustained anti-adherence activity of taurolidine (Taurolin) and noxythiolin (Noxyflex S) solutions J Pharm Pharmacol 40 509–511 Occurrence Handle2904994

    PubMed  Google Scholar 

  4. ND Bouvy RL Marquet J Jeekel HJ Bonjer (1997) ArticleTitleLaparoscopic surgery is associated with less tumour growth stimulation than conventional surgery: an experimental study [see comments] Br J Surg 84 358–361 Occurrence Handle1:STN:280:ByiB3s3ht1I%3D Occurrence Handle9117307

    CAS  PubMed  Google Scholar 

  5. HP Bruch O Schwandner TH Schiedeck UJ Roblick (1999) ArticleTitleActual standards and controversies on operative technique and lymph node dissection in colorectal cancer Langenbecks Arch Surg 384 167–175 Occurrence Handle1:STN:280:DyaK1M3lvVOhsA%3D%3D Occurrence Handle10328170

    CAS  PubMed  Google Scholar 

  6. P Calabresi FA Goulette JW Darnowski (2001) ArticleTitleTaurolidine: cytotoxic and mechanistic evaluation of a novel antineoplastic agent Cancer Res 61 6816–6821 Occurrence Handle1:CAS:528:DC%2BD3MXntFaisb0%3D Occurrence Handle11559556

    CAS  PubMed  Google Scholar 

  7. AA Conlan E Abramor P Delikaris SS Hurwitz (1983) ArticleTitleTaurolidine instillation as therapy for empyema thoracis. A prospective study of 50 patients S Afr Med J 64 653–655 Occurrence Handle1:STN:280:BiuD3MjptlM%3D Occurrence Handle6353621

    CAS  PubMed  Google Scholar 

  8. L Connolly P Maxwell (2002) ArticleTitleImage analysis of Transwell assays in the assessment of invasion by malignant cell lines Br J Biomed Sci 59 11–14 Occurrence Handle12000178

    PubMed  Google Scholar 

  9. ML Da Costa HP Redmond DJ Bouchier-Hayes (2001) ArticleTitleTaurolidine improves survival by abrogating the accelerated development and proliferation of solid tumors and development of organ metastases from circulating tumor cells released following surgery J Surg Res 101 111–119 Occurrence Handle1:CAS:528:DC%2BD3MXovFWisrY%3D Occurrence Handle11735264

    CAS  PubMed  Google Scholar 

  10. BM Ghadimi PM Schlag (1998) ArticleTitleTumor metastasis. Molecular principles and therapeutic options Chirurg 69 1315–1322 Occurrence Handle1:STN:280:DyaK1M7kslKgtg%3D%3D Occurrence Handle10023553

    CAS  PubMed  Google Scholar 

  11. SP Gorman DF McCafferty BJ Lewis (1987) ArticleTitleElectron and light microscopic observations of bacterial cell surface effects due to taurolidine treatment Lett Appli Microbiol 62 103–109

    Google Scholar 

  12. CN Gutt V Riemer ZG Kim CA Jacobi V Paolucci M Lorenz (1999) ArticleTitleImpact of laparoscopic colonic resection on tumour growth and spread in an experimental model Br J Surg 86 1180–1184 Occurrence Handle1:STN:280:DyaK1Mvjs1Klug%3D%3D Occurrence Handle10504374

    CAS  PubMed  Google Scholar 

  13. CA Jacobi J Ordemann KI Cuyper ParticleDe JM Müller (1999) ArticleTitleLaparoskopische Chirurgie maligner Tumoren. Experimentelle Ergebnisse zur Pathogenese und Prävention von Trokarmetastasen Viszeralchirurgie 34 182–189

    Google Scholar 

  14. CA Jacobi FJ Peter FA Wenger J Ordemann JM Muller (1999) ArticleTitleNew therapeutic strategies to avoid intra- and extraperitoneal metastases during laparoscopy: results of a tumor model in the rat Dig Surg 16 393–399 Occurrence Handle1:STN:280:DC%2BD3c%2Fjs12rug%3D%3D Occurrence Handle10567800

    CAS  PubMed  Google Scholar 

  15. CA Jacobi R Sabat J Ordemann F Wenger HD Volk JM Muller (1997) ArticleTitlePeritoneal instillation of taurolidine and heparin for preventing intraperitoneal tumor growth and trocar metastases in laparoscopic operations in the rat model Langenbecks Arch Chir 382 31–36

    Google Scholar 

  16. C Mavrantonis SD Wexner JJ Nogueras EG Weiss F Potenti AJ Pikarsky (2002) ArticleTitleCurrent attitudes in laparoscopic colorectal surgery Surg Endosc 16 1152–1157 Occurrence Handle1:STN:280:DC%2BD38vjt1ehsw%3D%3D Occurrence Handle12015620

    CAS  PubMed  Google Scholar 

  17. JW Milsom B Bohm KA Hammerhofer VW Fazio E Steiger P Elson (1998) ArticleTitleA prospective randomized trial comparing laparoscopic vs. conventional techniques in colorectal cancer surgery: a preliminary report J Am Coll Surg 187 46–54 Occurrence Handle1:STN:280:DyaK1czitFClsw%3D%3D Occurrence Handle9660024

    CAS  PubMed  Google Scholar 

  18. I Opitz HC Veen C Braumann B Ablassmaier K Fuhrer CA Jacobi (2003) ArticleTitleThe influence of adhesion prophylactic substances and taurolidine/heparin on local recurrence and intraperitoneal tumor growth after laparoscopic-assisted bowel resection of colon carcinoma in a rat model Surg Endosc 17 1098–1104 Occurrence Handle1:STN:280:DC%2BD2czos1Cnsw%3D%3D Occurrence Handle12712383

    CAS  PubMed  Google Scholar 

  19. HB Reith (1997) ArticleTitleTherapy of peritonitis today. Surgical management and adjuvant therapy strategies. Langenbecks Arch Chir 382 S14–S17 Occurrence Handle1:STN:280:ByiH3M7lt1E%3D

    CAS  Google Scholar 

  20. LA Repesh (1989) ArticleTitleA new in vitro assay for quantitating tumor cell invasion Invasion Metastasis 9 192–208 Occurrence Handle1:STN:280:BiaB2MvjsV0%3D Occurrence Handle2722434

    CAS  PubMed  Google Scholar 

  21. MA Reymond B Hu A Garcia T Reck F Kockerling J Hess Morel (2000) ArticleTitleFeasibility of therapeutic pneumoperitoneum in a large animal model using a microvaporisator Surg Endosc 14 51–55 Occurrence Handle1:STN:280:DC%2BD3c7hs12gtQ%3D%3D Occurrence Handle10653236

    CAS  PubMed  Google Scholar 

  22. MA Reymond C Schneider S Kastl W Hohenberger F Kockerling (1998) ArticleTitleThe pathogenesis of port-site recurrences J Gastrointest Surg 2 406–414 Occurrence Handle1:STN:280:DyaK1M%2FlvF2rtA%3D%3D Occurrence Handle9843599

    CAS  PubMed  Google Scholar 

  23. DP Shrayer H Lukoff T King Calabresi (2003) ArticleTitleThe effect of Taurolidine on adherent and floating subpopulations of melanoma cells Anticancer Drugs 14 295–303 Occurrence Handle1:CAS:528:DC%2BD3sXisF2itLk%3D Occurrence Handle12679734

    CAS  PubMed  Google Scholar 

  24. D Skipper AJ Cooper JE Marston I Taylor (1981) ArticleTitleExfoliated cells and in vitro growth in colorectal cancer Br J Surg 74 1049–1052

    Google Scholar 

  25. KH Staubach (1997) ArticleTitleAdjuvant therapy of peritonitis with taurolidine. Modulation of mediator liberation. Langenbecks Arch Chir 382 S26–S30 Occurrence Handle1:STN:280:ByiH3M7lt1w%3D

    CAS  Google Scholar 

  26. R Stendel L Scheurer G Stoltenburg-Didinger M Brock H Möhler (2003) ArticleTitleEnhancement of Fas-ligand-mediated programmed cell death by taurolidine Anticancer Res 23 2309–2314 Occurrence Handle1:CAS:528:DC%2BD3sXmsFyrt70%3D Occurrence Handle12894508

    CAS  PubMed  Google Scholar 

  27. R Stendel G Stoltenburg-Didinger CL Al Keikh M Wattrodt M Brock (2002) ArticleTitleThe effect of taurolidine on brain tumor cells Anticancer Res 22 809–814 Occurrence Handle1:CAS:528:DC%2BD38XktF2nu7w%3D Occurrence Handle12014655

    CAS  PubMed  Google Scholar 

  28. CB Walker DM Bruce SD Heys DB Gough NR Binnie O Eremin (1999) ArticleTitleMinimal modulation of lymphocyte and natural killer cell subsets following minimal access surgery Am J Surg 177 48–54 Occurrence Handle1:STN:280:DyaK1M7lsV2isA%3D%3D Occurrence Handle10037308

    CAS  PubMed  Google Scholar 

  29. Waser, PG, Kirsch, WM (1985) “Pharmakologie und Toxikologie von Taurolidin” In: Brückner, Pfirrmann, RW, Taurolidin: Ein neues konzept zur autiunilerobiellen chemotherapie chirurgischer Infelctionen, Urban und Schooltrenberg, pp 24–27

  30. SD Wexner SM Cohen (1995) ArticleTitlePort site metastases after laparoscopic colorectal surgery for cure of malignancy Br J Surg 82 295–298 Occurrence Handle1:STN:280:ByqA3Mnjs1w%3D Occurrence Handle7795990

    CAS  PubMed  Google Scholar 

  31. SM Willatts S Radford M Leitermann (1995) ArticleTitleEffect of the antiendotoxic agent, taurolidine, in the treatment of sepsis syndrome: a placebo-controlled, double-blind trial Crit Care Med 23 1033–1039 Occurrence Handle1:STN:280:ByqB1M3lsFE%3D Occurrence Handle7774213

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Surgery, Otto-von-Guericke University, Leipziger Strasse 44, D-39120, Magdeburg, Germany

    G. Nestler, H. U. Schulz, D. Schubert, H. Lippert & M. Pross

  2. Institute of Pathology, Otto-von-Guericke University, Leipziger Strasse 44, D-39120, Magdeburg, Germany

    S. Krüger

Authors
  1. G. Nestler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. U. Schulz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. Schubert
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Krüger
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. Lippert
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. Pross
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G. Nestler.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nestler, G., Schulz, H.U., Schubert, D. et al. Impact of taurolidine on the growth of CC531 coloncarcinoma cells in vitro and in a laparoscopic animal model in rats. Surg Endosc 19, 280–284 (2005). https://doi.org/10.1007/s00464-003-9301-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00464-003-9301-8

Keywords

Search

Navigation