Skip to main content
SpringerLink
Log in

The effects of aeration and agitation on the measurement of yeast biomass using a laser turbidity probe

  • Published:
Bioprocess Engineering Aims and scope Submit manuscript

Abstract

 The paper reports the measurement of biomass concentration using a laser turbidity probe. A suspension of Bakers’ yeast (0.5−50 gl-1) was subjected to various conditions of agitation and aeration in a stirred tank reactor and the turbidity measured using the probe. Both agitation and aeration were found to influence the turbidity.

At any constant biomass concentration, the effect on the turbidity measurement of changing agitation or aeration rate independently was linear, while at any constant conditions of agitation and aeration rate, the relationship between turbidity and biomass concentration was non-linear.

The results indicate that, in a bioprocess with non-particulate medium, it is possible to correct for the effects of aeration and agitation on turbidity measurement using a multivariate calibration model. A procedure for calibration and correction of measurements for the effects of agitation and aeration is presented and is verified using experimental data. This procedure may be generalised for other applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Centre for Process Systems Engineering, Imperial College of Science Technology and Medicine, London SW7 2BY, U.K. and Department of Chemical and Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, U.K., , , , , , GB

    M. E. Gregory

  2. Department of Electronic and Electrical Engineering, University College London, Torrington Place, London WC1E 7JE, U.K., , , , , , GB

    N. F. Thornhill

Authors
  1. M. E. Gregory
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. F. Thornhill
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Received: 29 January 1996

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gregory, M., Thornhill, N. The effects of aeration and agitation on the measurement of yeast biomass using a laser turbidity probe. Bioprocess Engineering 16, 339–344 (1997). https://doi.org/10.1007/s004490050332

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s004490050332

Keywords

Search

Navigation