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Enzyme-based sensing on nanohybrid film coated over FTO electrode for highly sensitive detection of antibiotics

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Abstract

Cefepime and Meropenem are the new class of antibiotics, which are particularly used as last potent defender or the antibiotics of the last resort against multi-resistant bacterial species. In this paper, an impedance-based electrochemical biosensor was fabricated for identifying antibiotics of last resort in the forensic samples including gastric lavage and other body fluids. The sensor was developed using platinum nanoparticles (PtNPs) and electrodeposited zinc oxide- zinc hexacyanoferrate hybrid film (ZnO/ZnHCF) on the surface of a fluorine-doped glass electrode (FTO). Further, penicillinase was immobilized onto the modified electrode using penicillinase enzyme. The developed biosensor exhibits a good analytical response for the detection of antibiotics evaluated using electrochemistry studies. The linear response of the fabricated electrode was observed from 0.1 to 750 µM and the electrode limit of detection (LOD) was observed as 0.1 µM. The sensor confirms good accuracy, is highly selective, and sensitive for the target. While storing the modified electrode at 4 °C, the stability of biosensor was evaluated for 45 days, and activity loss of 30–40% was observed. The highly sensitive interface of Penicillinase@CHIT/PtNP-ZnO/ZnHCF/FTO electrode shows a promising future in forensic studies.

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Acknowledgements

This work is financially supported by Extramural Research Grant (File No. EMR/2016/007564) and Young Scientist Scheme (File No. YSS/2015/000023) of Science and Engineering Research Board, Government of India, and Technology Development Program (TDP) (TDP/BDTD/33/2019) of Department of Science and Technology (DST), Government of India and Biotechnology Industry Research Assistance Council (BIRAC) (File No. BT/IIPME0211/02/16).

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Authors and Affiliations

  1. Amity Institute of Nanotechnology (AINT), Amity University Uttar Pradesh (AUUP), Noida, 201313, India

    Nidhi Chauhan, Sapna Balayan, Shaivya Gupta & Utkarsh Jain

  2. School of Allied Health Sciences (SAHS), Sharda University, Greater Noida, 201306, Uttar Pradesh, India

    Jaskaran Singh

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  1. Nidhi Chauhan
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  2. Sapna Balayan
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Correspondence to Utkarsh Jain.

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Chauhan, N., Balayan, S., Gupta, S. et al. Enzyme-based sensing on nanohybrid film coated over FTO electrode for highly sensitive detection of antibiotics. Bioprocess Biosyst Eng 44, 2469–2479 (2021). https://doi.org/10.1007/s00449-021-02618-3

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