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Magnetic fields exhibit a positive impact on lipid and biomass yield during phototrophic cultivation of Spirulina sp.

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Abstract

This study aimed to investigate the effects of magnetic field (MF) application (1, 12 and 24 h day −1) to Spirulina sp. LEB 18 in different photosynthesis cycles (dark and/or light) during short (15 days) and long periods (50 days) of cultivation. MF application was performed via two sources: ferrite magnets and solenoids. At the end of cultivation, the biomass was characterized in terms of lipids, proteins, and carbohydrates. In the 15 day cultures, the highest maximum biomass concentrations (2.06 g L−1 and 1.83 g L−1) were observed when 30 mT was applied for 24 h day −1 or 12 h day −1 (on the light cycle), respectively. MF application throughout cultivation (24 h day −1) for more than 30 days is not recommended. In all conditions, there was an increase in the lipid concentration (from 14 to 45%). The protein profile suggested important changes in photosystems I and II due to MF application. Cell morphology was not altered by MF application. In conclusion, the effects on the metabolism of Spirulina sp. are directly related to the photosynthesis cycle and time period in which the MF was applied.

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Funding

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES)-Finance Code 001 and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) Grant No 485575/2013-2.

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Authors and Affiliations

  1. Laboratory of Biotechnology, School of Chemistry and Food, Federal University of Rio Grande, Rio Grande, Rio Grande do Sul, 96203-900, Brazil

    Bruno da Costa Menestrino, Luisa Sala & Lucielen Oliveira Santos

  2. Laboratory of Biochemical Engineering, School of Chemistry and Food, Federal University of Rio Grande, Rio Grande, Rio Grande do Sul, 96203-900, Brazil

    Jorge Alberto Vieira Costa

  3. Laboratory Mycotoxins and Food Science, School of Chemistry and Food, Federal University of Rio Grande, Rio Grande, Rio Grande do Sul, 96203-900, Brazil

    Jaqueline Garda Buffon

Authors
  1. Bruno da Costa Menestrino
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  2. Luisa Sala
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  4. Jaqueline Garda Buffon
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  5. Lucielen Oliveira Santos
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Contributions

Material preparation, data collection, analysis, investigation, writing—original draft, review and editing and visualization were performed by BCM. Writing—review and editing was performed by LS. Supervision and writing—review and editing was performed by JAVC. Methodology, supervision and writing—review and editing were performed by JGB. Conceptualization, writing—review and editing, methodology, supervision, project administration and funding acquisition were performed by LOS. All authors read and approved the final manuscript.

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Correspondence to Lucielen Oliveira Santos.

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da Costa Menestrino, B., Sala, L., Costa, J.A.V. et al. Magnetic fields exhibit a positive impact on lipid and biomass yield during phototrophic cultivation of Spirulina sp.. Bioprocess Biosyst Eng 44, 2087–2097 (2021). https://doi.org/10.1007/s00449-021-02585-9

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