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High production of optically pure (3R)-acetoin by a newly isolated marine strain of Bacillus subtilis CGMCC 13141

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Abstract

Acetoin is one of the bio-based platform chemicals and its optically pure isomers are important potential intermediates and precursors in the synthesis of novel optically active materials. (3R)-acetoin could be synthesized via enzymatic catalysis, whole-cell catalysis and fermentation. In this study a marine strain of Bacillus subtilis was isolated to produce optically pure (3R)-acetoin with glucose as carbon source. The effects of nutrients on the formation of (3R)-acetoin and conversion of glucose to (3R)-acetoin were evaluated by Plackett–Burman design, and the fermentation medium was optimized by central composite design. The impact of oxygen supply on the production of (3R)-acetoin was studied at different aeration rates. Under the optimal conditions, 83.7 g/L (3R)-acetoin with an optical purity of 99.4% was achieved by fed-batch fermentation, and the conversion of glucose to (3R)-acetoin was 91.5% of the theoretical value. The results indicate the industrial potential of this strain for (3R)-acetoin production via fermentation.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant no. 21476042).

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  1. School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, People’s Republic of China

    Jianying Dai, Zhuangfei Wang & Zhi-Long Xiu

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  1. Jianying Dai
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  2. Zhuangfei Wang
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Correspondence to Zhi-Long Xiu.

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Dai, J., Wang, Z. & Xiu, ZL. High production of optically pure (3R)-acetoin by a newly isolated marine strain of Bacillus subtilis CGMCC 13141. Bioprocess Biosyst Eng 42, 475–483 (2019). https://doi.org/10.1007/s00449-018-2051-8

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