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Performance of Bacillus subtilis on fibrous biomass sugar hydrolysates in producing biosurfactants and techno-economic comparison

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Abstract

Surfactin and fatty acetyl glutamate (FA-Glu) were produced by Bacillus subtilis in 5-L fermentor. In a previous 50-mL shake flask study, sugar hydrolysates from soy hull, alfalfa and switchgrass were shown to support the growth of Bacillus strains. It was observed that glucose content and availability of hexose and pentose sugars in the hydrolysates played an important role in determining growth and product concentration. Growth, economic efficiency and product concentration of biosurfactants was compared in fermentations conducted in 5-L stirred tank bioreactor, on biomass hydrolysate-based growth media. Highest bacterial growth absorbance for surfactin and FA-Glu producing strains were at 3.5 and 3.3 absorbance units, respectively, for switchgrass hydrolysate media. Highest concentrations of products were observed in soy hull hydrolysate media (2.9 g/L and 0.28 g/L for surfactin and FA-Glu). Techno-economic analysis of the 5-L fermentations on the three biomasses showed surfactin cost estimate to be $6.63/kg for 97% pure product.

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Authors and Affiliations

  1. Food Science and Human Nutrition, Iowa State University, 536 Farm House Ln, Ames, IA, 50011, USA

    Rajat Sharma & Buddhi P. Lamsal

  2. Mechanical Engineering, Iowa State University, 2078 Black Engineering, Ames, IA, 50011, USA

    Mark Mba-Wright

Authors
  1. Rajat Sharma
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  2. Buddhi P. Lamsal
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  3. Mark Mba-Wright
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Correspondence to Buddhi P. Lamsal.

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Sharma, R., Lamsal, B.P. & Mba-Wright, M. Performance of Bacillus subtilis on fibrous biomass sugar hydrolysates in producing biosurfactants and techno-economic comparison. Bioprocess Biosyst Eng 41, 1817–1826 (2018). https://doi.org/10.1007/s00449-018-2004-2

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  • DOI: https://doi.org/10.1007/s00449-018-2004-2

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