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Process development of oxalic acid production in submerged culture of Aspergillus niger F22 and its biocontrol efficacy against the root-knot nematode Meloidogyne incognita

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Abstract

Oxalic acid has potent nematicidal activity against the root-knot nematode Meloidogyne incognita. In this study, fermentation parameters for oxalic acid production in submerged culture of Aspergillus niger F22 at 23, 25, and 30 °C were optimized in 5-L jar fermenters. The viscosity of the culture broth increased with increasing temperature. There was a negative correlation between oxalic acid production and the apparent viscosity; high volumetric productivity of oxalic acid was obtained at low apparent viscosity (less than 1000 cP), with a productivity of more than 100 mg/L h. When the apparent viscosity was over 2500 cP, the volumetric productivity decreased below 50 mg/L h. In addition, the volumetric mass transfer coefficient, K L a, positively correlated with volumetric productivity. When the K L a value increased from 0.0 to 0.017 /s, the volumetric productivity proportionally increased up to 176 mg/L h. When the temperature decreased, K L a increased due to the decrease in viscosity, leading to increased volumetric productivity. The highest productivity of 7453.3 mg/L was obtained at the lowest temperature, i.e., 23 °C. The nematicidal activity of culture filtrate was proportional to the content of oxalic acid. Based on a constant impeller tip speed, oxalic acid production was successfully scaled up to a 500-L pilot vessel, producing a final concentration comparable to that in the 5-L jar.

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Acknowledgements

This research was supported by grants from the Rural Development Administration (PJ01020703) and the World Institute of Kimchi (KE1701-1), funded by the Ministry of Science, ICT, and Future Planning, Republic of Korea.

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Authors and Affiliations

  1. R&D Division, World Institute of Kimchi, Gwangju, 61755, Republic of Korea

    Sang Il Lee, Keon Jin Lee, Ho Hyun Chun, Sanghyun Ha, Hyun Jung Gwak, Ho Myeong Kim, Jong-Hee Lee, Hak-Jong Choi & Hae Woong Park

  2. National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju, Jeonbuk, 55365, Republic of Korea

    Hyeong Hwan Kim

  3. Farm Hannong, Nonsan, Chungnam, 33010, Republic of Korea

    Teak Soo Shin

  4. Division of Applied Bioscience and Biotechnology, Chonnam National University, Gwangju, 61186, Republic of Korea

    Jin-Cheol Kim

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  1. Sang Il Lee
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Correspondence to Hae Woong Park or Jin-Cheol Kim.

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Lee, S.I., Lee, K.J., Chun, H.H. et al. Process development of oxalic acid production in submerged culture of Aspergillus niger F22 and its biocontrol efficacy against the root-knot nematode Meloidogyne incognita . Bioprocess Biosyst Eng 41, 345–352 (2018). https://doi.org/10.1007/s00449-017-1867-y

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