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Insights into the simultaneous utilization of glucose and glycerol by Streptomyces albulus M-Z18 for high ε-poly-l-lysine productivity

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Abstract

The simultaneous consumption of glucose and glycerol led to remarkably higher productivity of both biomass and ε-poly-l-lysine (ε-PL), which was of great significance in industrial microbial fermentation. To further understand the superior fermentation performances, transcriptional analysis and exogenous substrates addition were carried out to study the simultaneous utilization of glucose and glycerol by Streptomyces albulus M-Z18. Transcriptome analysis revealed that there was no mutual transcriptional suppression between the utilization of glucose and glycerol, which was quite different from typical “glucose effect”. In addition, microorganisms cultivated with single glycerol showed significant demand for ribose-5-phosphate, which resulted in potential demand for glucose and xylitol. The above demand could be relieved by glucose (in the mixed carbon source) or xylitol addition, leading to improvement of biomass production. It indicated that glucose in the mixed carbon source was more important for biomass production. Besides, transcriptional analysis and exogenous citrate addition proved that single carbon sources could not afford enough carbon skeletons for Embden Meyerhof pathway (EMP) while a glucose–glycerol combination could provided sufficient carbon skeletons to saturate the metabolic capability of EMP, which contributed to the replenishment of precursors and energy consumed in ε-PL production. This study offered insight into the simultaneous consumption of glucose and glycerol in the ε-PL batch fermentation, which deepened our comprehension on the high ε-PL productivity in the mixed carbon source.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (31301556), the Fundamental Research Funds for the Central Universities (JUSRP51504), the Open Project Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China (KLIBKF201302), and the Jiangsu Province Collaborative Innovation Center for Advanced Industrial Fermentation Industry Development Program.

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Authors and Affiliations

  1. College of Life Sciences, Huaibei Normal University, Huaibei, 235000, China

    Xin Zeng

  2. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China

    Xin Zeng, Junjie Zhao, Xusheng Chen & Zhonggui Mao

  3. Family Planning Service Center, Rizhao Maternal and Child Care Service Hospital, Rizhao, 276826, China

    Wenyun Miao

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  1. Xin Zeng
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  2. Junjie Zhao
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  3. Xusheng Chen
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  4. Zhonggui Mao
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Correspondence to Xusheng Chen or Zhonggui Mao.

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Zeng, X., Zhao, J., Chen, X. et al. Insights into the simultaneous utilization of glucose and glycerol by Streptomyces albulus M-Z18 for high ε-poly-l-lysine productivity. Bioprocess Biosyst Eng 40, 1775–1785 (2017). https://doi.org/10.1007/s00449-017-1832-9

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  • DOI: https://doi.org/10.1007/s00449-017-1832-9

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