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The role of NdgR in glycerol metabolism in Streptomyces coelicolor

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Abstract

Streptomyces, which produces many pharmaceutical antibiotics and anticancer agents, is a genus of soil-dwelling bacteria with numerous regulators that control both primary and secondary metabolism. NdgR is highly conserved in Streptomyces spp. and is known to be involved in antibiotic production, tolerance against shock and physical stress, nitrogen metabolism, leucine metabolism, and N-acetylglucosamine metabolism. As another function of NdgR, we report the involvement of NdgR in glycerol metabolism in S. coelicolor. Initially, a glycerol utilization operon containing gylCABX was found to be up-regulated in an ndgR deletion mutant (BG11) grown in N-acetylglucosamine solid minimal media compared with wild-type strain (M145). BG11 produced more antibiotics with a small amount of glycerol and increased glycerol utilization, yielding higher concentrations of lactate and acetate per cell. Moreover, fatty acid production was also changed in BG11 to produce longer chain fatty acids, phenolic compounds, alkanes, and fatty alcohols. Using a gel retardation assay, NdgR was found to bind the upstream region of gylC, working as a repressor. NdgR is a second regulator of a glycerol utilization operon, for which only one regulator, GylR was previously known.

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Acknowledgements

Consulting service from the Microbial Carbohydrate Resource Bank (MCRB, Seoul, Korea) was kindly appreciated.

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Authors and Affiliations

  1. Department of Microbial Engineering, College of Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea

    Bo-Rahm Lee, Shashi Kant Bhatia, Hun-Suk Song, Junyoung Kim, Hyung-yeon Park & Yung-Hun Yang

  2. Institute for Ubiquitous Information Technology and Applications (UBITA), Konkuk University, Seoul, 143-701, Republic of Korea

    Shashi Kant Bhatia & Yung-Hun Yang

  3. Division of Infectious Diseases, Rhode Island Hospital, Alpert Medical School of Brown University, Providence, RI, USA

    Wooseong Kim

  4. Intelligent Sustainable Materials R&D Group, Korea Institute of Industrial Technology (KITECH), Chungnam, 31056, Republic of Korea

    Jeong-Jun Yoon

  5. School of Chemical and Biological Engineering, Seoul National University, Kwanak-gu, Seoul, 151-742, Republic of Korea

    Sung-Hee Park & Byung-Gee Kim

  6. Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, Republic of Korea

    Daehee Hwang

  7. Center for Plant Aging Research, Institute for Basic Science, DGIST, Daegu, Republic of Korea

    Daehee Hwang

Authors
  1. Bo-Rahm Lee
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  2. Shashi Kant Bhatia
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  3. Hun-Suk Song
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  4. Junyoung Kim
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  5. Wooseong Kim
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  6. Hyung-yeon Park
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  7. Jeong-Jun Yoon
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  8. Sung-Hee Park
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  9. Daehee Hwang
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  10. Byung-Gee Kim
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  11. Yung-Hun Yang
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Corresponding author

Correspondence to Yung-Hun Yang.

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Funding

The study was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2017R1D1A1B03033594 and NRF-2015M1A5A1037196), the Korea Institute of Energy Technology Evaluation and Planning (KETEP), and the Ministry of Trade, Industry and Energy (MOTIE) of the Republic of Korea (No. 20163010092150). This study was also partially supported by the Advanced Production Technology Development Program, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea (1201349190011).

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The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Lee, BR., Bhatia, S.K., Song, HS. et al. The role of NdgR in glycerol metabolism in Streptomyces coelicolor . Bioprocess Biosyst Eng 40, 1573–1580 (2017). https://doi.org/10.1007/s00449-017-1813-z

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  • DOI: https://doi.org/10.1007/s00449-017-1813-z

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