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Graphene nanoflakes on transparent glass electrode sensor for electrochemical sensing of anti-diabetic drug

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Abstract

Metformin (Mf) plays a major role in controlling insulin level of individuals at risk of developing diabetes mellitus. Overdose of Mf can cause lactic acidosis, diarrhoea, cough, or hoarseness, etc. These particulars point out the identification for selective and sensitive methods of Mf determination. In the present work, graphene nanoflakes-polymethylene blue (GNF-PMB) nano-composites were developed onto fluorine-doped tin oxide (SnO2/F) coated glass substrates for electrochemical sensing of Mf using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The developed sensor shows quick response time (10 s), linearity as 10–103 µM, LOD (0.1 nM), and good shelf life (10 weeks). Attempts have been made to utilize this electrode for estimation of Mf in urine samples. Configured as a highly responsive, reproducible Mf sensor, it combines the electrical properties of GNF and stable electron transfer of PMB. The newly developed Mf sensor presents a promising candidate in point-of-care diagnosis.

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Acknowledgements

The present work was supported by one of the authors (Jagriti Narang) by SERB, Department of Science and Technology (DST), (Grant Number SERB/LS-962/2012) India. Authors are highly thankful to Dr. N. Vijyan, Sr. Scientist, NPL, New Delhi, for providing X-ray diffraction facility. Thanks to all scientists referenced throughout the paper whose valuable work has guided the way through to this research work.

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Authors and Affiliations

  1. Amity Institute of Nanotechnology (AINT), AMITY University, Noida, 201313, Uttar Pradesh, India

    Jagriti Narang, Chaitali Singhal, Rishabh Bhatia, Vikas Kathuria & Manan Jain

  2. Amity Institute of Physiotherapy, AMITY University, Noida, 201313, Uttar Pradesh, India

    Nitesh Malhotra

Authors
  1. Jagriti Narang
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  2. Nitesh Malhotra
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  3. Chaitali Singhal
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  4. Rishabh Bhatia
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  5. Vikas Kathuria
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  6. Manan Jain
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Correspondence to Jagriti Narang.

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Narang, J., Malhotra, N., Singhal, C. et al. Graphene nanoflakes on transparent glass electrode sensor for electrochemical sensing of anti-diabetic drug. Bioprocess Biosyst Eng 40, 537–548 (2017). https://doi.org/10.1007/s00449-016-1719-1

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  • DOI: https://doi.org/10.1007/s00449-016-1719-1

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