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A newly isolated and identified vitamin B12 producing strain: Sinorhizobium meliloti 320

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Abstract

Vitamin B12 (Cobalamin, VB12) has several physiological functions and is widely used in pharmaceutical and food industries. A new unicellular species was extracted from China farmland, and the strain could produce VB12 which was identified by HPLC and HPLC–MS/MS. 16S rDNA analysis reveals this strain belongs to the species Sinorhizobium meliloti and we named it S. meliloti 320. Its whole genome information indicates that this strain has a complete VB12 synthetic pathway, which paves the way for further metabolic engineering studies. The optimal carbon and nitrogen sources are sucrose and corn steep liquor (CSL) plus peptone. The optimal combination of sucrose and CSL was obtained by response surface methodology as they are the most suitable carbon and nitrogen sources, respectively. This strain could produce 140 ± 4.2 mg L−1 vitamin B12 after incubating for 7 days in the optimal medium.

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Acknowledgments

We are grateful for the support from State Key Development Program for Basic Research of China (973 Program, 2013CB733600), National Nature Science Foundation of China (31370089) and Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences.

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    Authors and Affiliations

    1. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China

      Huina Dong, Sha Li, Huan Fang, Miaomiao Xia, Ping Zheng, Dawei Zhang & Jibin Sun

    2. Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People’s Republic of China

      Huina Dong, Huan Fang, Miaomiao Xia, Ping Zheng, Dawei Zhang & Jibin Sun

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    1. Huina Dong
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    2. Sha Li
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    3. Huan Fang
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    4. Miaomiao Xia
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    5. Ping Zheng
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    Corresponding authors

    Correspondence to Ping Zheng or Dawei Zhang.

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    H. Dong, S. Li are contributed equally to this work.

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    Dong, H., Li, S., Fang, H. et al. A newly isolated and identified vitamin B12 producing strain: Sinorhizobium meliloti 320. Bioprocess Biosyst Eng 39, 1527–1537 (2016). https://doi.org/10.1007/s00449-016-1628-3

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    • DOI: https://doi.org/10.1007/s00449-016-1628-3

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