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Influence of oxygen transfer on Pseudomonas putida effects on growth rate and biodesulfurization capacity

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Abstract

The growth rate and desulfurization capacity accumulated by the cells during the growth of Pseudomonas putida KTH2 under different oxygen transfer conditions in a stirred and sparged tank bioreactor have been studied. Hydrodynamic conditions were changed using different agitation conditions. During the culture, several magnitudes associated to growth, such as the specific growth rate, the dissolved oxygen concentration and the carbon source consumption have been measured. Experimental results indicate that cultures are influenced by the fluid dynamic conditions into the bioreactor. An increase in the stirrer speed from 400 to 700 rpm has a positive influence on the cell growth rate. Nevertheless, the increase of agitation from 700 to 2000 rpm hardly has any influence on the growth rate. The effect of fluid dynamics on the cells development of the biodesulfurization (BDS) capacity of the cells during growth is different. The activities of the intracellular enzymes involved in the 4S pathway change with dissolved oxygen concentration. The enzyme activities have been evaluated in cells at several growth time and different hydrodynamic conditions. An increase of the agitation from 100 to 300 rpm has a positive influence on the development of the overall BDS capacity of the cells during growth. This capacity shows a decrease for higher stirrer speeds and the activity of the enzymes monooxygenases DszC and DszA decreases dramatically. The highest value of the activity of DszB enzyme was obtained with cells cultured at 100 rpm, while this activity decreases when the stirrer speed was increased higher than this value.

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Abbreviations

a j :

Activity of enzyme j \( \left( \rm{\mu M \ g_{{C_{X} }}^{ - 1} \ min^{ - 1} } \right) \)

BDS:

Biodesulfurisation

C* :

Oxygen concentration in equilibrium (mol L−1)

Cj :

Concentration of compound j \( \left( \rm{\mu M \ L^{ - 1} } \right) \)

CO2 :

Oxygen dissolved concentration (% or mol·m−3)

C X :

Concentration of biomass \( \left( {g_{X} \cdot L^{ - 1} } \right) \)

D:

Stirrer diameter (m)

DBT:

Dibenzothiophene

DBTO:

Dibenzothiophene sulfoxide

DBTO2 :

Dibenzothiophene-sulfone

F:

Fischer statistical parameter

HBP:

2-Hydroxybiphenyl

HBPS:

2-Hydroxybiphenyl-2-sulfinic acid

k L a :

Volumetric oxygen mass transfer coefficient (s−1)

N:

Stirrer speed (rpm)

OTR:

Oxygen transfer rate (mol O2L−1s−1)

OUR:

Oxygen uptake rate (mol O2L−1s−1)

Re :

Impeller Reynolds number (–)

SSR:

Sum of square residuals

T :

Vessel diameter (m)

t :

Time (min, s or h)

V :

Volume (m3)

Xj :

Conversion or yield of compound j (–)

η :

Effectiveness factor for growth (–)

µ :

Specific growth rate (h−1)

G :

Relative to gas phase

L :

Relative to liquid phase

Max:

Referred to maximum value

O2 :

Referred to oxygen

X :

Referred to biomass

0:

Referred to initial value

t :

Referred to time of 120 min of resting cell assay

0:

Referred to initial value

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Acknowledgments

This work has been supported by MICINN under contracts CTQ2013-45970-C2-1-R, CTQ2011-12725-E and BSCH-UCM, GR35/10-A 910134.

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Authors and Affiliations

  1. Chemical Engineering Department, Faculty of Chemistry, Universidad Complutense de Madrid, 28040, Madrid, Spain

    S. Escobar, A. Rodriguez, E. Gomez, A. Alcon, V. E. Santos & Felix Garcia-Ochoa

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  1. S. Escobar
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  2. A. Rodriguez
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  3. E. Gomez
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Correspondence to Felix Garcia-Ochoa.

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Escobar, S., Rodriguez, A., Gomez, E. et al. Influence of oxygen transfer on Pseudomonas putida effects on growth rate and biodesulfurization capacity. Bioprocess Biosyst Eng 39, 545–554 (2016). https://doi.org/10.1007/s00449-016-1536-6

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  • DOI: https://doi.org/10.1007/s00449-016-1536-6

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