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Bioconversion of Iminodiacetonitrile to Iminodiacetic acid with whole cells of Lysinibacillus boronitolerans MTCC 107614 (IICT-akl252)

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Abstract

Biotechnological potential of nitrilases are prompting significant interest in finding the novel microbes capable of hydrolyzing nitriles. In this view, we have screened about 450 bacterial strains for nitrilase production using bioconversion of iminodiacetonitrile (IDAN) to iminodiacetic acid (IDA) through hydrolysis and obtained six nitrilase-producing isolates. Among these six isolates, IICT-akl252 was promising which was identified as Lysinibacillus boronitolerans. This is the first report on L. boronitolerans for nitrilase activity. Optimization of various medium and reaction parameters for maximizing the nitrilase production using whole cells in shake flask was carried out for L. boronitolerans IICT-akl252. Sucrose (2 %) as a carbon source attained better nitrilase yield while IDAN appeared to be the preferable inducer (0.2 %). The maximum IDA formation was achieved with 100 mM IDAN and 150 mg/ml cells at 30 °C and pH 6.5. After optimization of the culture and reaction conditions, the activity of nitrilase was increased by 2.3-fold from 27.2 to 64.5 U. The enzyme was stable up to 1 h at 50 °C. The enzyme was able to hydrolyze aliphatic, aromatic and heterocyclic nitrile substrates.

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Acknowledgments

The authors acknowledge Council of Scientific and Industrial Research (CSIR), New Delhi, for the financial assistance provided to Hemalatha in the form of a Senior Research Fellowship. The authors thank Dr. Suman Kumar Mamidyala for his kind help during the preparation of the manuscript. The authors also acknowledge CSIR for financial support under the 12th Five Year plan project “Affordable Cancer Therapeutics (ACT)” (CSC0301).

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Authors and Affiliations

  1. Medicinal Chemistry and Pharmacology, Indian Institute of Chemical Technology, Hyderabad, 500 607, India

    Hemalatha Muluka, Gurrala Sheelu & Y. V. D. Nageshwar

  2. Department of Biotechnology, Acharya Nagarjuna University, Guntur, India

    Hemalatha Muluka

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  1. Hemalatha Muluka
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  2. Gurrala Sheelu
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  3. Y. V. D. Nageshwar
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Correspondence to Y. V. D. Nageshwar.

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Muluka, H., Sheelu, G. & Nageshwar, Y.V.D. Bioconversion of Iminodiacetonitrile to Iminodiacetic acid with whole cells of Lysinibacillus boronitolerans MTCC 107614 (IICT-akl252). Bioprocess Biosyst Eng 39, 413–420 (2016). https://doi.org/10.1007/s00449-015-1524-2

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