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Starch based polyhydroxybutyrate production in engineered Escherichia coli

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Abstract

Every year, the amount of chemosynthetic plastic accumulating in the environment is increasing, and significant time is required for decomposition. Bio-based, biodegradable plastic is a promising alternative, but its production is not yet a cost effective process. Decreasing the production cost of polyhydroxyalkanoate by utilizing renewable carbon sources for biosynthesis is an important aspect of commercializing this biodegradable polymer. An Escherichia coli strain that expresses a functional amylase and accumulate polyhydroxybutyrate (PHB), was constructed using different plasmids containing the amylase gene of Panibacillus sp. and PHB synthesis genes from Ralstonia eutropha. This engineered strain can utilize starch as the sole carbon source. The maximum PHB production (1.24 g/L) was obtained with 2 % (w/v) starch in M9 media containing 0.15 % (w/v) yeast extract and 10 mM glycine betaine. The engineered E. coli SKB99 strain can accumulate intracellular PHB up to 57.4 % of cell dry mass.

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Acknowledgments

The authors have declared no conflict of interest. This work was partially supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2013R1A1A2A10004690), Korea Polar Research Institute (PE14030) and the Energy Efficiency and Resources of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea Government Ministry of Trade, Industry and Energy (20133030000300). This subject is also supported by the Korea Ministry of Environment as a “Converging Technology Project (201-101-007)” and as an “Eco-Innovation Project (405-112-0382).” In addition, this research was supported by the 2014 KU Brain Pool of Konkuk University.

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Authors and Affiliations

  1. Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, South Korea

    Shashi Kant Bhatia, Young-Ha Shim, Jong-Min Jeon, Yong-Hyun Kim, Hyun-Joong Kim, Hyung-Min Seo, Ju-Hee Lee, Jung-Ho Kim, Da-Hye Yi & Yung-Hun Yang

  2. Department of Bioengineering, University of Massachusetts Dartmouth, 285 Old Westport Road, North Dartmouth, MA, 02747-2300, USA

    Christopher J. Brigham

  3. Division of Life Sciences, Korea Polar Research Institute, 12 Gaetbeol-ro, Yeonsu-gu, Incheon, 406-840, South Korea

    Yoo Kyung Lee

  4. Institute for Ubiquitous Information Technology and Applications (CBRU), Konkuk University, Seoul, 143-701, South Korea

    Yung-Hun Yang

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  1. Shashi Kant Bhatia
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  2. Young-Ha Shim
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  3. Jong-Min Jeon
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  4. Christopher J. Brigham
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  5. Yong-Hyun Kim
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  7. Hyung-Min Seo
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  8. Ju-Hee Lee
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  9. Jung-Ho Kim
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  11. Yoo Kyung Lee
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Correspondence to Yung-Hun Yang.

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Bhatia, S.K., Shim, YH., Jeon, JM. et al. Starch based polyhydroxybutyrate production in engineered Escherichia coli . Bioprocess Biosyst Eng 38, 1479–1484 (2015). https://doi.org/10.1007/s00449-015-1390-y

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  • DOI: https://doi.org/10.1007/s00449-015-1390-y

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