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Customized optimization of cellulase mixtures for differently pretreated rice straw

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Abstract

Lignocellulose contains a large amount of cellulose but is recalcitrant to enzymatic hydrolysis, which yields sugars for fuels or chemicals. Various pretreatment methods are used to improve the enzymatic digestibility of cellulose in lignocellulose. Depending on the lignocellulose types and pretreatment methods, biomass compositions and physical properties significantly vary. Therefore, customized enzyme mixtures have to be employed for the efficient hydrolysis of pretreated lignocellulose. Here, using three recombinant model enzymes consisting of endoglucanase, cellobiohydrolase, and xylanase with a fixed amount of β-glucosidase, the optimal formulation of enzyme mixtures was designed for two differently pretreated rice straws (acid-pretreated or alkali-pretreated rice straw) by the mixture design methodology. As a result, different optimal compositions for the enzyme mixtures were employed depending on the type of pretreatment of rice straw. These results suggest that customized enzyme mixtures for pretreated lignocellulosic biomass are necessary to obtain increased sugar yields and should be considered in the industrial utilization of lignocellulose.

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Acknowledgments

This work was supported by Grants from the National Research Foundation of Korea (2013M1A2A2072597) and the Ministry of Trade, Industry and Energy (10049674). Experiments were performed at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety.

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Authors and Affiliations

  1. Department of Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea

    In Jung Kim, Ju Yeon Jung, Hee Jin Lee, Hyong Seok Park, Young Hoon Jung & Kyoung Heon Kim

  2. Department of Biological and Chemical Engineering, Hongik University, Sejong, 399-701, Republic of Korea

    Kyungmoon Park

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  1. In Jung Kim
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Correspondence to Kyoung Heon Kim.

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Kim, I.J., Jung, J.Y., Lee, H.J. et al. Customized optimization of cellulase mixtures for differently pretreated rice straw. Bioprocess Biosyst Eng 38, 929–937 (2015). https://doi.org/10.1007/s00449-014-1338-7

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  • DOI: https://doi.org/10.1007/s00449-014-1338-7

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