Abstract
In recent decades, the production of compounds from microorganisms has increased significantly. Glycerol as a source of substrate appears to have great potential, due to its large supply because of the increase in biodiesel production. This paper will discuss the multiplicity of steady states for the production of 1,3-propanediol from glycerol by Clostridium butyricum, employing a model that takes into account inhibition by fermentation products. The theoretical study of bifurcation enabled us to make a qualitative adjustment to the various experimental steady states, using the theoretical steady states obtained from the AUTO2007 program. The theoretical model parameters were varied to fit qualitatively the values of the experimental steady states. In addition, this work is a qualitative study, using experimental steady states that can be used as an initial study for more advanced work on optimizing the production of 1,3-propanediol.
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Abbreviations
- X :
-
Biomass concentration (mmol/L)
- C s :
-
Substrate concentration (mmol/L)
- μ :
-
Specific growth rate (per h)
- D :
-
Dilution rate (per h)
- C sf :
-
Substrate concentration in feed (mmol/L)
- q s :
-
Specific rate of substrate uptake (mmol g/L)
- C p :
-
Product concentration (mmol/L)
- C pp :
-
1,3-propanediol concentration (mmol/L)
- C paa :
-
Acetic acid concentration (mmol/L)
- C pab :
-
Butyric acid concentration (mmol/L)
- q p :
-
Specific rate of product formation (mmol g/L)
- μ max :
-
Maximum specific growth rate (per h)
- K s :
-
Monod saturation constant (mmol/L)
- C * :
-
Maximal concentration (mmol/L)
- m :
-
Maintenance term of substrate consumption and product formation under substrate-limited conditions (mmol g/L)
- Y m :
-
Maximum growth yield and product yield (mmol g)
- Δq m :
-
Maximum substrate consumption rate and product formation rate under substrate-sufficient conditions (mmol/L per h)
- K * :
-
Saturation constants for substrate and product in kinetic equations with excess terms (mmol/L)
- V :
-
Volume of bioreactor (L)
- P :
-
Production (g/h)
- F :
-
Outflow (L/h)
- s:
-
Substrate glycerol
- pp:
-
Product 1,3-propanediol
- paa:
-
Product acetic acid
- pab:
-
Product butyric acid
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Silva, J.P., Almeida, Y.B., Pinheiro, I.O. et al. Multiplicity of steady states in a bioreactor during the production of 1,3-propanediol by Clostridium butyricum . Bioprocess Biosyst Eng 38, 229–235 (2015). https://doi.org/10.1007/s00449-014-1262-x
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DOI: https://doi.org/10.1007/s00449-014-1262-x