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Mercury sensing and toxicity studies of novel latex fabricated silver nanoparticles

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Abstract

Safe and eco-friendly alternatives to currently used hazardous chemico-physical methods of silver nanoparticles (AgNPs) synthesis are need of time. Rapid, low cost, selective detection of toxic metals in environmental sample is important to take safety action. Toxicity assessment of engineered AgNPs is essential to avoid its side effects on human and non-target organisms. In the present study, biologically active latex from Euphorbia heterophylla (Poinsettia) was utilized for synthesis of AgNPs. AgNPs was of spherical shape and narrow size range (20–50 nm). Occurrence of elemental silver and crystalline nature of AgNPs was analyzed. Role of latex metabolites in reduction and stabilization of AgNPs was analyzed by FT-IR, protein coagulation test and phytochemical analysis. Latex-synthesized AgNPs showed potential in selective and sensitive detection of toxic mercury ions (Hg2+) with limit of detection around 100 ppb. Addition of Hg2+ showed marked deviation in color and surface plasmon resonance spectra of AgNPs. Toxicity studies on aquatic non-target species Daphnia magna showed that latex-synthesized AgNPs (20.66 ± 1.52 % immobilization) were comparatively very less toxic than chemically synthesized AgNPs (51.66 ± 1.52 % immobilization). Similarly, comparative toxicity study on human red blood cells showed lower hemolysis (4.46 ± 0.01 %) by latex-synthesized AgNPs as compared to chemically synthesized AgNPs causing 6.14 ± 0.01 % hemolysis.

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Acknowledgments

Hemant P. Borase is a DST-INSPIRE fellow (Grant File No. DST/INSPIRE Fellowship/2011[149]). Chandrashekhar D. Patil is thankful to CSIR (Ref: 09/728 (0028)/2012-EMR-I) for the award of senior research fellowship.

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Authors and Affiliations

  1. School of Life Sciences, North Maharashtra University, Post Box 80, Jalgaon, 425001, Maharashtra, India

    Hemant P. Borase, Chandrashekhar D. Patil, Rahul B. Salunkhe, Rahul K. Suryawanshi, Bipinchandra K. Salunke & Satish V. Patil

  2. North Maharashtra Microbial Culture Collection Centre (NMCC), North Maharashtra University, Post Box 80, Jalgaon, 425001, Maharashtra, India

    Satish V. Patil

Authors
  1. Hemant P. Borase
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  2. Chandrashekhar D. Patil
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  3. Rahul B. Salunkhe
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  4. Rahul K. Suryawanshi
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  5. Bipinchandra K. Salunke
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  6. Satish V. Patil
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Corresponding author

Correspondence to Satish V. Patil.

Electronic supplementary material

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449_2014_1200_MOESM1_ESM.tif

Supplementary 1(a) UV–Visible absorption of latex synthesized AgNPs (A) and chemically synthesized AgNPs (B) (b) Eppendorf tubes showing- intact latex (1), AgNPs synthesized by intact latex (2), Aqueous fraction of latex (3), AgNPs synthesized by aqueous fraction (4), Non-aqueous fraction of latex (5) and AgNPs synthesized by non-aqueous fraction (6) and UV–Visible absorption of AgNPs using- Rub- Rubber (non-aqueous fraction), Whole- intact latex and Aq- aqueous fraction of latex. (TIFF 814 kb)

Supplementary 2 (DOC 35 kb)

449_2014_1200_MOESM3_ESM.tif

Supplementary 3 (a) Control filter paper (A), AgNPs impregnated filter paper (B) and AgNPs impregnated filter paper dipped in Hg2+ solution (C), Photograph showing  % Hemolysis assay, red blood cells treated with- Distilled water (positive control (1). PBS saline as negative control (2), 10 % latex (3), Latex synthesized AgNPs (4) and chemically synthesized AgNPs (5) (b). (TIFF 539 kb)

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Borase, H.P., Patil, C.D., Salunkhe, R.B. et al. Mercury sensing and toxicity studies of novel latex fabricated silver nanoparticles. Bioprocess Biosyst Eng 37, 2223–2233 (2014). https://doi.org/10.1007/s00449-014-1200-y

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  • DOI: https://doi.org/10.1007/s00449-014-1200-y

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