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Physico-chemical characterization and biological response of Labeo rohita-derived hydroxyapatite scaffold

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Abstract

The chemically treated Labeo rohita scale is used for synthesizing hydroxyapatite (HAp) biomaterials. Thermogravimetric and differential thermal analyses of fish scale materials reveal the different phase changes with temperature and find out the suitable calcination temperatures. The composition and structures of wet ball-milled calcined HAp powders are characterized by Fourier transform infrared spectroscopy, X-ray diffraction, field emission scanning electron microscopy, transmission electron microscopy, energy dispersive X-ray analysis (EDX). The EDX as well as chemical analysis of fish scale-derived apatite materials confirms that the Ca/P ratio is 1.71. The compressive stress, hardness and porosity have been evaluated on sintered HAp biomaterials. The cell attachment on HAp surfaces, cytotoxicity evaluation and MTT assay, which are carried out in RAW macrophage-like cell line media demonstrate good biocompatibility. The histological analysis also supports the bioaffinity of processed HAp biomaterials in Wistar rat model for investigating the contact reaction and stability at the artificial or natural prosthesis interface.

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Acknowledgments

The authors would like to express their gratitude to Director, CSIR-CMERI for his kind permission to publish this paper. The authors are thankful to Dr. Syamal Roy, Head of the department of Immunology and infectious diseases at IICB Kolkata for their kind support for cell culture, toxicity studies. The authors are also indebted to CSIR-Centre for Cellular and Molecular Biology (CSIR-CCMB), Hyderabad for histological and in vivo studies. The financial support from CSIR is highly acknowledged.

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Authors and Affiliations

  1. Centre for Advanced Materials Processing, CSIR-Central Mechanical Engineering Research Institute, Mahatma Gandhi Avenue, Durgapur, 713 209, India

    S. Mondal, N. Mandal & B. Mondal

  2. Department of Biotechnology, Heritage Institute of Technology, Kolkata, India

    A. Mondal

  3. Department of Biotechnology, National Institute of Technology, Durgapur, 713 209, India

    S. S. Mukhopadhyay & A. Dey

  4. CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India

    S. Singh

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  1. S. Mondal
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  2. A. Mondal
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  3. N. Mandal
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  4. B. Mondal
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Correspondence to B. Mondal.

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Mondal, S., Mondal, A., Mandal, N. et al. Physico-chemical characterization and biological response of Labeo rohita-derived hydroxyapatite scaffold. Bioprocess Biosyst Eng 37, 1233–1240 (2014). https://doi.org/10.1007/s00449-013-1095-z

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  • DOI: https://doi.org/10.1007/s00449-013-1095-z

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