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Reversible immobilization of invertase on Cu-chelated polyvinylimidazole-grafted iron oxide nanoparticles

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Abstract

Polyvinylimidazole (PVI)-grafted iron oxide nanoparticles (PVIgMNP) were prepared by grafting of telomere of PVI on the iron oxide nanoparticles. Different metal ions (Cu2+, Zn2+, Cr2+, Ni2+) ions were chelated on polyvinylimidazole-grafted iron oxide nanoparticles, and then the metal-chelated magnetic particles were used in the adsorption of invertase. The maximum invertase immobilization capacity of the PVIgMNP–Cu2+ beads was observed to be 142.856 mg/g (invertase/PVIgMNP) at pH 5.0. The values of the maximum reaction rate (V max) and Michaelis–Menten constant (Km) were determined for the free and immobilized enzymes. The enzyme adsorption–desorption studies, pH effect on the adsorption efficiency, affinity of different metal ions, the kinetic parameters and storage stability of free and immobilized enzymes were evaluated.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Arts and Sciences, Fatih University, Buyukcekmece Kampusu, 34500, B.Cekmece, Istanbul, Turkey

    Kerem Uzun, Mehmet Şenel & Abdülhadi Baykal

  2. Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, 34500, B.Cekmece, Istanbul, Turkey

    Emre Çevik

Authors
  1. Kerem Uzun
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  2. Emre Çevik
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  3. Mehmet Şenel
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  4. Abdülhadi Baykal
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Correspondence to Mehmet Şenel.

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Uzun, K., Çevik, E., Şenel, M. et al. Reversible immobilization of invertase on Cu-chelated polyvinylimidazole-grafted iron oxide nanoparticles. Bioprocess Biosyst Eng 36, 1807–1816 (2013). https://doi.org/10.1007/s00449-013-0955-x

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  • DOI: https://doi.org/10.1007/s00449-013-0955-x

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