Skip to main content
SpringerLink
Log in

Approaching chlorpyrifos bioelimination at bench scale bioreactor

  • Original Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

Chlorpyrifos (CP) is one of the most commonly applied insecticides for control of pests and insects. The inappropriate use of this kind of chemicals has caused heavy contamination of many terrestrial and aquatic ecosystems thus representing a great environmental and health risk. The main purpose of this work is to investigate novel microbial agents (Pseudomonas stutzeri and the previously obtained consortium LB2) with the ability to degrade CP from polluted effluents. This goal was achieved by operating at different lab scales (flask and bioreactor) and operation modes (batch and fed-batch). Very low degradation and biomass levels were detected in cultures performed with the consortium LB2. In contrast, near complete CP degradation was reached by P. stutzeri at the optimal conditions in less than 1 month, showing a depletion rate of 0.054 h−1. The scale-up at bench scale stirred tank bioreactor allowed improving the specific degradation rate in ten folds and total CP degradation was obtained after 2 days. Moreover, biomass and biodegradation profiles were modelled to reach a better characterization of the bioremediation process.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Rubio V, Ferere A (2005) Control biológico de plantas y enfermedades de los cultivos. En Biotecnología y Medioambiente, Ephemera

    Google Scholar 

  2. Fulekar MH (2010) Environmental biotechnology. CRC Press, Enfield

    Google Scholar 

  3. Anwar S, Liaquat F, Khan QM, Khalid ZM, Iqbal S (2009) Biodegradation of chlorpyrifos and its hydrolysis product 3,5,6-trichloro-2-pyridinol by Bacillus pumilus strain C2A1. J Hazard Mater 168:400–405

    Article  CAS  Google Scholar 

  4. Latifi AM, Khodi S, Mirzaei M, Miresmaeili M, Babavalian H (2012) Isolation and characterization of five chlorpyrifos degrading bacteria. Afr J Biotechnol 11:3140–3146

    CAS  Google Scholar 

  5. Dubey KK, Fulekar MH (2012) Chlorpyrifos bioremediation in Pennisetum rhizosphere by a novel potential degrader Stenotrophomona smaltophilia MHF ENV20. Word J Microbiol Biotechnol 28:1715–1725

    Article  CAS  Google Scholar 

  6. Singh BK, Walker A (2006) Microbial degradation of organophosphorus compounds. FEMS Microbiol Rev 30:428–471

    Article  CAS  Google Scholar 

  7. Vidya-Lakshmi C, Kumar M, Khanna S (2008) Biotransformation of chlorpyrifos and bioremediation of contaminated soil. Int Biodeterior Biodegr 62:204–209

    Article  Google Scholar 

  8. Lakshmi CV, Kumar M, Khanna S (2009) Biodegradation of chlorpyrifos in soil by enriched cultures. Curr Microbiol 58:35–38

    Article  Google Scholar 

  9. Pino N, Peñuela G (2011) Simultaneous degradation of the pesticides methyl parathion and chlorpyrifos by an isolated bacterial consortium from a contaminated site. Int Biodeterior Biodegrad 65:827–831

    Article  CAS  Google Scholar 

  10. Maya K, Singh RS, Upadhyay SN, Dubey SK (2011) Kinetic analysis reveals bacterial efficacy for biodegradation of chlorpyrifos and its hydrolyzing metabolite TCP. Process Biochem 46:2130–2136

    Article  CAS  Google Scholar 

  11. Rauh VA, Perera FP, Horton MK, Whyatt RM, Bansal R, Hao XJ, Liu J, Barr DB, Slotkin TA, Peterson BS (2012) Brain anomalies in children exposed prenatally to a common organophosphate pesticide. Proc Natl Acad Sci USA 109:7871–7876

    Article  CAS  Google Scholar 

  12. Mallick K, Bharati K, Banerji A, Shakil NA, Sethunathan N (1999) Bacterial degradation of chlorpyrifos in pure cultures andin soil. B Environ Contam Toxicol 62:48–54

    Article  CAS  Google Scholar 

  13. Singh BK, Walker A, Morgan JAW, Wright DJ (2003) Effects of soil pH on the biodegradation of chlorpyrifos and isolation of a chlorpyrifos-degrading bacterium. Appl Environ Microbiol 69:5198–5206

    Article  CAS  Google Scholar 

  14. Singh BK, Walker A, Morgan JAW, Wright DJ (2004) Biodegradation of chlorpyrifos by Enterobacter strain B-14 and its use in bioremediation of contaminated soils. Appl Environ Microbiol 70:4855–4863

    Article  CAS  Google Scholar 

  15. Yang L, Zhao YH, Zhang BX, Yang CH, Zhang X (2005) Isolation and characterization of a chlorpyrifos and 3,5,6-trichloro-2-pyridinol degrading bacterium. FEMS Microbiol Lett 251:67–73

    Article  CAS  Google Scholar 

  16. Ghanem I, Orfi M, Shamma M (2007) Biodegradation of chlorpyrifos by Klebsiella sp.isolated from an activated sludge sample of waste water treatment plant in Damascus. Folia Microbiol 52:423–427

    Article  CAS  Google Scholar 

  17. Singh DP, Khattar JIS, Nadda J, Singh Y, Garg A, Kaur N, Gulati A (2011) Chlorpyrifos degradation by the cyanobacterium Synechocystis sp. strain PUPCCC 64. Environ Sci Pollut Res 18:1351–1359

    Article  CAS  Google Scholar 

  18. Zhao H, Wu Q, Wang L, Zhao X, Gao H (2009) Degradation of phenanthrene by bacterial strain isolated from soil in oil refinery fields in Shanghai China. J Hazard Mater 164:863–869

    Article  CAS  Google Scholar 

  19. Moscoso F, Deive FJ, Longo MA, Sanromán MA (2012) Technoeconomic assessment of phenanthrene degradation by Pseudomonas stutzeri CECT 930 in a batch bioreactor. Bioresour Technol 104:81–89

    Article  CAS  Google Scholar 

  20. Deive FJ, Carvalho E, Pastrana L, Rua ML, Longo MA, Sanromán MA (2009) Assessment of relevant factors influencing lipolytic enzyme production by Thermus thermophilus HB27 in laboratory-scale bioreactors. Chem Eng Technol 32:606–612

    Article  CAS  Google Scholar 

  21. Deive FJ, Dominguez A, Barrio T, Moscoso F, Morán P, Longo MA, Sanromán MA (2010) Decolorization of dye Reactive Black 5 by newly isolated thermophilic microorganisms from geothermal sites in Galicia (Spain). J Hazard Mater 182:735–742

    Article  CAS  Google Scholar 

  22. Seo JS, Keum YS, Harada RM, Li QX (2007) Isolation and characterization of bacteria capable of degrading Polycyclic Aromatic Hydrocarbons (PAHs) and organophosphorus pesticides from PAH-contaminated soil in Hilo, Hawaii. J Agric Food Chem 55:5383–5389

    Article  CAS  Google Scholar 

  23. Bautista LF, Sanz R, Molina MC, González N, Sánchez D (2009) Effect of different non-ionic surfactants on the biodegradation of PAHs by diverse aerobic bacteria. Int Biodeterior Biodegrad 63:913–922

    Article  Google Scholar 

  24. Caceres T, He W, Naidu R, Megharaj M (2007) Toxicity of chlorpyrifos and TCP alone and in combination to Daphnia carinata: the influence of microbial degradation in natural water. Water Res 41:4497–4503

    Article  CAS  Google Scholar 

  25. Marques AM, Estanol I, Alsina JM, Fuste C, Simonpujol D, Guinea J, Congregado F (1986) Production and rheological properties of the extracellular polysaccharide synthesized by Pseudomonas sp. strain EPS-5028. Appl Environ Microbiol 52:1221–1223

    CAS  Google Scholar 

  26. Deive FJ, López E, Rodríguez A, Longo MA, Sanromán MA (2012) Targeting the production of biomolecules by extremophiles at bioreactor scale. Chem Eng Technol 35:1565–1575

    Article  CAS  Google Scholar 

  27. Mathava K, Ligy P (2007) Biodegradation of endosulfan-contaminated soil in a pilot-scale reactor-bioaugmented with mixed bacterial culture. J Environ Sci Health B 42:707–715

    Google Scholar 

  28. Venkata-Mohan S, Sirisha K, Sreenivasa Rao R, Sarma PN (2007) Bioslurry phase remediation of chlorpyrifos contaminated soil: process evaluation and optimization by Taguchi design of experimental (DOE) methodology. Ecotoxicol Environ Safe 68:252–262

    Article  CAS  Google Scholar 

  29. Venkata-Mohan S, Sirisha K, Chandrasekhara Rao N, Sarma PN, Jayarama SR (2004) Degradation of chlorpyrifos contaminated soil by bioslurry reactor operated in sequencing batch mode: bioprocess monitoring. J Hazard Mater 116:39–48

    Article  Google Scholar 

  30. Ghoshdastidar AJ, Saunders JE, Brown KH, Tong AZ (2012) Membrane bioreactor treatment of commonly used organophosphate pesticides. J Environ Sci Health B 47:742–750

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was funded by Spanish Ministry of Science and Innovation (Project CTQ2008-0359). The authors are grateful to Xunta de Galicia for financial support of Francisco Deive under the Parga Pondal program.

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, University of Vigo, 36310, Vigo, Spain

    F. Moscoso, I. Teijiz, F. J. Deive & M. A. Sanromán

Authors
  1. F. Moscoso
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. Teijiz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. J. Deive
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. A. Sanromán
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. A. Sanromán.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Moscoso, F., Teijiz, I., Deive, F.J. et al. Approaching chlorpyrifos bioelimination at bench scale bioreactor. Bioprocess Biosyst Eng 36, 1303–1309 (2013). https://doi.org/10.1007/s00449-012-0876-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-012-0876-0

Keywords

Search

Navigation