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d-Tagatose production in the presence of borate by resting Lactococcus lactis cells harboring Bifidobacterium longum l-arabinose isomerase

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Abstract

Bifidobacterium longum NRRL B-41409 l-arabinose isomerase (l-AI) was overexpressed in Lactococcus lactis using a phosphate depletion inducible expression system. The resting L. lactis cells harboring the B. longum l-AI were used for production of d-tagatose from d-galactose in the presence of borate buffer. Multivariable analysis suggested that high pH, temperature and borate concentration favoured the conversion of d-galactose to d-tagatose. Almost quantitative conversion (92 %) was achieved at 20 g L−1 substrate and at 37.5 °C after 5 days. The d-tagatose production rate of 185 g L−1 day−1 was obtained at 300 g L−1 galactose, at 1.15 M borate, and at 41 °C during 10 days when the production medium was changed every 24 h. There was no significant loss in productivity during ten sequential 24 h batches. The initial d-tagatose production rate was 290 g L−1 day−1 under these conditions.

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Acknowledgments

Auli Murrola is thanked for analyzing a countless number of HPLC samples.

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Authors and Affiliations

  1. Department of Biotechnology and Chemical Technology, Aalto University, P.O. Box 16100, 00076, Aalto, Finland

    Noora Salonen, Kalle Salonen & Matti Leisola

  2. VTT Technical Research Centre of Finland, P.O. Box 1000, 02044, Espoo, Finland

    Antti Nyyssölä

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  1. Noora Salonen
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  2. Kalle Salonen
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  3. Matti Leisola
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  4. Antti Nyyssölä
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Correspondence to Noora Salonen.

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Salonen, N., Salonen, K., Leisola, M. et al. d-Tagatose production in the presence of borate by resting Lactococcus lactis cells harboring Bifidobacterium longum l-arabinose isomerase. Bioprocess Biosyst Eng 36, 489–497 (2013). https://doi.org/10.1007/s00449-012-0805-2

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  • DOI: https://doi.org/10.1007/s00449-012-0805-2

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