Abstract
We describe cloning and characterization of three rice (Oryza sativa) NADPH-cytochrome P450 reductases (OsCPRs; E.C.1.6.2.4) that are potential donors to plant P450s, including tryptamine 5-hydroxylase (T5H) in serotonin synthesis and cinnamate 4-hydroxylase (C4H) in phenylpropanoid synthesis. All three OsCPR transcripts are induced to varying degrees by stresses. Co-expression of full-length OsCPR1, OsCPR2 and OsCPR3 with either T5H or C4H in E. coli indicated that the OsCPR2/T5H and OsCPR2/C4H constructs displayed the highest T5H and C4H catalytic activities. The N-terminal residues of OsCPR2 were required for peak electron transfer activity to P450 even though deletion mutants with short N-terminal deletions were capable of reducing cytochrome c.
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This research was supported by grants from the Basic Science Research Program (2009-0087207), the Priority Research Centers Program (2012-0005857) as well as the Chonnam National University (2010).
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Park, S., Kim, YS., Rupasinghe, S.G. et al. Rice P450 reductases differentially affect P450-mediated metabolism in bacterial expression systems. Bioprocess Biosyst Eng 36, 325–331 (2013). https://doi.org/10.1007/s00449-012-0787-0
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DOI: https://doi.org/10.1007/s00449-012-0787-0