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Production and separation of dipeptidyl peptidase IV from Lactococcus lactis: scale up for industrial production

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Abstract

Lactococcus lactis spp. lactis and Lactococcus lactis spp. cremoris are widely used in the manufacture of fermented milk. These strains were compared for production of Dipeptidyl Peptidase IV (DPP IV) enzyme in terms of enzyme activity, specific growth rates and productivity. Lactococcus lactis spp. lactis was produced in 3 L bioreactor and scaled up to 30 and 150 L stirred tank bioreactors, and the enzyme activities were found as 110, 110 and 122 mU mL−1, respectively. After 8 h of production, separation steps were performed. While purification fold was 127 and yield was 2.69 %, the molecular weight of the enzyme was estimated as 68 kDa. Partially purified enzyme was enteric coated with capsules and a 95.5 % of DPP IV enzyme passed into the artificial intestine. Results show that production of DPP IV enzyme by Lactococcus lactis spp. lactis strain in submerged culture is comparable with the productions by commercial strains, mostly Aspergillus, in solid state fermentations based on productivity.

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Abbreviations

CNS:

Central nervous system

CSL:

Corn steep liquor

CSLM:

Corn steep liquor mixture

DPP IV:

Dipeptidyl peptidase IV

D i :

Diameter of impeller

μ:

Dynamic viscosity

ε:

Energy dissipation

HPMCP:

Hydroxypropyl methylcellulose phthalate

h:

Hour

L:

Liter

mL:

Milliliter

mU:

Milliunit

MW:

Molecular weight

n :

The rotational speed of impeller

N P :

Power number

OD:

Optical density

P/V :

Volumetric power input

P :

Stirring power

Re i :

Reynolds number

R f :

Retention factor

ν:

Impeller-tip speed

υ:

Kinematic viscosity

ρ:

Liquid density

τ:

Shear stress

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Acknowledgments

This study was supported by The Scientific and Technological Research Council of Turkey (TUBİTAK) with file number 105T195. We are grateful to Professor Erdal Bedir for his advice and valuable support, and Professor Fazilet Vardar Sukan for her guidance.

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Authors and Affiliations

  1. Department of Food Engineering, Engineering Faculty, Pamukkale University, 20020, Kinikli, Denizli, Turkey

    Özlem Üstün

  2. Bioengineering Department, Faculty of Engineering, EBILTEM Binasi, Ege University, 35100, Bornova, Izmir, Turkey

    Gaye Öngen

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  1. Özlem Üstün
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Correspondence to Özlem Üstün.

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Üstün, Ö., Öngen, G. Production and separation of dipeptidyl peptidase IV from Lactococcus lactis: scale up for industrial production. Bioprocess Biosyst Eng 35, 1417–1427 (2012). https://doi.org/10.1007/s00449-012-0730-4

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  • DOI: https://doi.org/10.1007/s00449-012-0730-4

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