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Bi-stage control of dissolved oxygen to enhance cyclic adenosine monophosphate production by Arthrobacter A302

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Abstract

Experiments confirmed dissolved oxygen (DO) definitely affects cyclic adenosine monophosphate (cAMP) production by Arthrobacter A302. Production of cAMP by batch fermentation was investigated under various DO conditions. A two-stage DO control strategy was proposed to achieve optimal production of cAMP based on the kinetic analysis: the DO level was controlled at 40% during the first 18 h and then switched to 30%. Relatively high cAMP production (9.9 g L−1) was achieved by applying this strategy. The cAMP productivity (0.14 g L−1 h−1) was also successfully improved by 85.1, 59.3, 15.1 and 28.0%, compared to cases in which DO was uncontrolled or DO levels were held at 20, 30 and 40%, respectively. This is the first report of the use of a two-stage DO control strategy in cAMP production, and it was verified to be an effective method for enhancing the cAMP yield via this strain.

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Acknowledgments

This work was supported by the Major Basic R & D Program of China (2009CB724700), China National Funds for Distinguished Young Scientists (21025625), Program for Changjiang Scholars and Innovative Research Team in University and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Authors and Affiliations

  1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 210009, People’s Republic of China

    Lei Li, Xiaochun Chen, Jian Cheng, Chunwei Zhang, Jianxin Bai, Yong Chen, Huanqin Niu & Hanjie Ying

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  1. Lei Li
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  2. Xiaochun Chen
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  3. Jian Cheng
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  4. Chunwei Zhang
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  5. Jianxin Bai
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  6. Yong Chen
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  7. Huanqin Niu
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  8. Hanjie Ying
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Corresponding author

Correspondence to Hanjie Ying.

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L. Li and X. Chen contributed equally to this study.

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Li, L., Chen, X., Cheng, J. et al. Bi-stage control of dissolved oxygen to enhance cyclic adenosine monophosphate production by Arthrobacter A302. Bioprocess Biosyst Eng 35, 1281–1286 (2012). https://doi.org/10.1007/s00449-012-0715-3

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  • DOI: https://doi.org/10.1007/s00449-012-0715-3

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