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Significance of protein elicitor isolated from Tuber melanosporum on the production of ganoderic acid and Ganoderma polysaccharides during the fermentation of Ganoderma lucidum

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Abstract

Under the elicitation of protein elicitor isolated from the culture mycelia of Tuber melanosporum, the biosynthesis of ganoderic acids (GA) was significantly stimulated during Ganoderma lucidum fermentation. Compared with our previous results that, GA content was inhibited by polysaccharide elicitor isolated from T. melanosporum, while improved by the elicitor of polysaccharide and protein, protein was identified to be the exact component inducing GA biosynthesis in this work. G. lucidum cell growth was significantly inhibited by elicitor of polysaccharide and protein, and polysaccharide elicitor did not inhibit the cell growth. In this work, the remarkable inhibition on the cell growth was considerably eliminated under the elicitation of protein elicitor isolated from T. melanosporum. These suggested maybe the interaction of polysaccharide and protein components existed in the inhibition on the cell growth of G. lucidum. Not only GA content but also total GA accumulation obtained the highest values after the elicitation of protein elicitor. The maximal GA production of 260.5 ± 5.6 mg/L was 31.2% higher than the control. Under the elicitation of protein elicitor, the production of extracellular polysaccharide (EPS) and the content of intracellular polysaccharide (IPS) were also enhanced; however, total IPS accumulation was lower. GA biosynthesis was also significantly affected by the addition time of protein elicitor, whose optimal value was the culture of day 4.

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Acknowledgments

Financial support from the National Natural Science Foundation of China (NSFC, Project Nos. 20706012 and 20976038), the National High Technology Research and Development Key Program of China (863 Key Program, 2007AA021506), National Basic Research Program of China (973 Program, 2007CB714306), the Key Project of Chinese Ministry of Education, Hubei Provincial Natural Science Foundation for Innovative Research Team (Project No. 2008CDA002), Discipline Leader Project of Wuhan Municipality (Project No. 200951830553), Scientific Research Key Project of Hubei Provincial Department of Education and the Open Project Program for Key Laboratory of Fermentation Engineering (Ministry of Education) are gratefully acknowledged. Ya-Jie Tang also thanks the Chutian Scholar Program from Hubei Provincial Department of Education, China (2006).

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  1. Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan, 430068, China

    Li-Wen Zhu & Ya-Jie Tang

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  1. Li-Wen Zhu
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Correspondence to Ya-Jie Tang.

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Zhu, LW., Tang, YJ. Significance of protein elicitor isolated from Tuber melanosporum on the production of ganoderic acid and Ganoderma polysaccharides during the fermentation of Ganoderma lucidum . Bioprocess Biosyst Eng 33, 999–1005 (2010). https://doi.org/10.1007/s00449-010-0424-8

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  • DOI: https://doi.org/10.1007/s00449-010-0424-8

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