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Adsorption and separation of proteins by a smectitic clay mineral

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Abstract

The adsorption of proteins by a smectitic clay mineral was investigated. The clay used in this study is a mixture of montmorillonite and amorphous SiO2. Due to the high porosity the montmorillonite units are accessible for protein adsorption. The amorphous silica prevents the montmorillonite from swelling and allows column packing. Protein adsorption was performed at different pH under static conditions. Furthermore, static capacities were determined. The material reveals high adsorption capacities for proteins under static conditions (270–408 mg/g), whereby proteins are mainly adsorbed via electrostatic interactions. The Freundlich isotherm is suggested as an adsorption model. For desorption a pH shift was found to be most effective. Binding and elution of human serum albumin and ovalbumin were tested under dynamic conditions. Dynamic capacities of about 40 mg/g for ovalbumin at 764 cm/h were found. The clay mineral provides suitable properties for the application as cost-efficient, alternative separation material.

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Authors and Affiliations

  1. Institut für Technische Chemie, Gottfried Wilhelm Leibniz Universität Hannover, Callinstraße 5, 30167, Hanover, Germany

    Kathrin Ralla, Daniel Riechers, Cornelia Kasper & Thomas Scheper

  2. Süd-Chemie AG, Ostenrieder Straße 15, 85368, Moosburg, Germany

    Ulrich Sohling & Friedrich Ruf

Authors
  1. Kathrin Ralla
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  2. Ulrich Sohling
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  3. Daniel Riechers
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  4. Cornelia Kasper
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  5. Friedrich Ruf
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  6. Thomas Scheper
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Correspondence to Thomas Scheper.

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Ralla, K., Sohling, U., Riechers, D. et al. Adsorption and separation of proteins by a smectitic clay mineral. Bioprocess Biosyst Eng 33, 847–861 (2010). https://doi.org/10.1007/s00449-010-0408-8

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  • DOI: https://doi.org/10.1007/s00449-010-0408-8

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