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Process optimization for the production of diosgenin with Trichoderma reesei

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Abstract

Based on the response surface methodology, an effective microbial system for diosgenin production from enzymatic pretreated Dioscorea zingiberensis tubers with Trichoderma reesei was studied. The fermentation medium was optimized with central composite design (35) depended on Plackett–Burmann design which identified significant impacts of peptone, K2HPO4 and Tween 80 on diosgenin yield. The effects of different fermentation conditions on diosgenin production were also studied. Four parameters, i.e. incubation period, temperature, initial pH and substrate concentration were optimized using 45 central composite design. The highest diosgenin yield of 90.57% was achieved with 2.67% (w/v) of peptone, 0.29% (w/v) of K2HPO4, 0.73% (w/v) of Tween 80 and 9.77% (w/v) of substrate, under the condition of pH 5.8, temperature 30 °C. The idealized incubation time was 6.5 days. After optimization, the product yield increased by 33.70% as compared to 67.74 ± 1.54% of diosgenin yield in not optimized condition. Scale-up fermentation was carried out in a 5.0 l bioreactor, maximum diosgenin yield of 90.17 ± 3.12% was obtained at an aeration of 0.80 vvm and an agitation rate of 300 rpm. The proposed microbial system is clean and effective for diosgenin production and thus more environmentally acceptable than the traditional acid hydrolysis.

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Acknowledgments

This work was supported by the National “11th-5-Year” Plan Project of China under Grant 2006BAB04A14.

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Authors and Affiliations

  1. The Key Laboratory of Water and Sediment Sciences, Ministry of Education, 100871, Beijing, China

    Yuling Zhu & Jinren Ni

  2. Department of Environmental Engineering, Peking University, 100871, Beijing, China

    Yuling Zhu & Jinren Ni

  3. Institute for Nanobiomedical Technology and Membrane Biology, State Key Laboratory of Biotherapy of Human Diseases, Cancer Center, West China Hospital, West China Medical School, Sichuan University, Keyuan 4 Lu, Gaoxinqu, 610041, Chengdu, China

    Wen Huang

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  1. Yuling Zhu
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  2. Jinren Ni
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Correspondence to Jinren Ni.

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Zhu, Y., Ni, J. & Huang, W. Process optimization for the production of diosgenin with Trichoderma reesei . Bioprocess Biosyst Eng 33, 647–655 (2010). https://doi.org/10.1007/s00449-009-0390-1

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