Skip to main content
SpringerLink
Log in

Enzymatic production of linalool esters in organic and solvent-free system

  • Original Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

This work investigated the influence of temperature, enzyme concentration, substrates molar ratio, in the absence and presence of organic solvent, at two molar ratios of the substrates on the enzymatic production of linalil esters using the immobilized lipase Novozym 435 as catalyst, different acids and linalool and Ho-Sho essential oil as substrates. The best reaction conversion was obtained at the highest temperature (70 °C), for both solvent free (3.81%) and with solvent addition (2.25%), for a solvent to substrates molar ratio of 2:1, enzyme concentration of 5 wt% and acid to alcohol molar ratio of 1:1. The reaction kinetics revealed that Ho-Sho essential oil afforded the greatest conversions when compared with pure linalool. Higher linalil esters production were achieved after 10 h reaction (5.58%) in 2:1 solvent to substrates molar ratio, with enzyme concentration of 5 wt%, at 70 °C and anhydride to alcohol molar ratio of 1:1 using Ho-Sho essential oil as substrate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Bauer M, Lei CK, Read R et al (2001) Phys Rev Lett 87:25501

    Article  Google Scholar 

  2. http://www.leffingwell.com/top_10.htm Accessed 24 April 2009

  3. Vandamme EJ, Soetaert W (2002) J Chem Technol Biotechnol 77:1323

    Article  CAS  Google Scholar 

  4. Krings U, Berger RG (1998) Appl Microbiol Biotechnol 49:222

    Article  Google Scholar 

  5. Melo LLMM, Pastore GM, Macedo GA (2005) Process Biochem 40:3181

    Article  CAS  Google Scholar 

  6. Melo LLMM, Pastore GM, Macedo GA (2005) Food Sci Biotechnol 14:368

    Google Scholar 

  7. Letizia CS, Cocchiara J, Lalko J et al (2003) Food Chem Toxicol 41:943

    Article  CAS  Google Scholar 

  8. Frizzo CD, Santos AC, Paroul N et al (2000) Braz Arch Biol Technol 43:313

    Article  CAS  Google Scholar 

  9. Novo Nordisk (1992), Technical Report A-05948

  10. Lanza M, Priamo WL, Oliveira JV et al (2004) Appl Biochem Biotechnol 113:181

    Article  Google Scholar 

  11. Silva DD, Chierice GO, Galhiane MS et al Química Nova 26: 461

  12. Castro HF, Anderson W, Moo-Young M et al (1992) Prog Biotechnol 8:476

    Google Scholar 

  13. Olsen T, Kerton F, Marriott R et al (2006) Enzyme Microb Technol 39:621

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Programa de Pós-Graduação em Biotecnologia, Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil

    Natália Paroul & Débora de Oliveira

  2. Universidade Regional Integrada do Alto Uruguai e das Missões, URI, Campus de Erechim, Av. Sete de Setembro, 1621, 99700-000, Erechim, RS, Brazil

    Natália Paroul, Adriana Biasi, Ane Cheila Rovani, Camile Prigol, Rogerio Dallago, Helen Treichel, Rogério L. Cansian, J. Vladimir Oliveira & Débora de Oliveira

Authors
  1. Natália Paroul
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Adriana Biasi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ane Cheila Rovani
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Camile Prigol
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rogerio Dallago
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Helen Treichel
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Rogério L. Cansian
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. J. Vladimir Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Débora de Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Helen Treichel.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Paroul, N., Biasi, A., Rovani, A.C. et al. Enzymatic production of linalool esters in organic and solvent-free system. Bioprocess Biosyst Eng 33, 583–589 (2010). https://doi.org/10.1007/s00449-009-0384-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-009-0384-z

Keywords

Search

Navigation